WRKY70 interacting with RCY1 disease resistance protein is required for resistance to Cucumber mosaic virus in Arabidopsis thaliana
WRKY70 interacting with RCY1 disease resistance protein is required for resistance to Cucumber mosaic virus in Arabidopsis thaliana
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DOI：
10.1016/j.pmpp.2013.11.001
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发表时间：
2014-01-01
影响因子：
2.7
通讯作者：
Takahashi, Hideki
中科院分区：
文献类型：
--
作者：
Ando, Sugihiro;Obinata, Atsuya;Takahashi, Hideki
CMV（Y）抗性基因（RCY 1）编码卷曲螺旋（CC）核苷酸结合（NB）富含亮氨酸重复序列（LRR）类抗性蛋白，其赋予对黄瓜花叶病毒[CMV（Y）]的黄色株系的抗性，并在病毒接种的拟南芥叶片中发展过敏反应（HR）细胞死亡。为了了解RCY 1介导的病毒防御反应，在酵母双杂交系统中使用CC-NB结构域片段作为诱饵分离与RCY 1的CC和NB结构域相互作用的宿主蛋白。WRKY 70转录因子可重复地与CC-NB结构域片段结合。在共免疫纯化实验中证实了WRKY 70和CC-NB结构域之间的这种相互作用，表明WRKY 70可能在RCY 1赋予的对CMV的抗性中起作用（Y）。在携带RCY 1的野生型植物的CMV（Y）接种的叶片中，病毒增殖受到抑制，并且WRKY 70和致病相关1（PR-I）转录物的水平明显增加。另一方面，在CMV（Y）接种的RCY 1转化的缺乏WRKY 70转录物的wrky 70 -1突变体的叶片中，病毒外壳蛋白积累显著增加，并且响应于CMV（Y）的PR-1表达的诱导部分受损。然而，在携带RCY 1的wrky 70 -1和野生型植物之间HR细胞死亡的程度没有显著差异。这些结果表明，WRKY 70可能在抑制病毒增殖中发挥关键作用，但不是在阐述HR细胞死亡，通过直接与RCY 1的CC-NB结构域相互作用。(C)2013爱思唯尔有限公司保留所有权利。
The gene RESISTANCE TO CMV(Y) (RCY1) encodes a coiled-coil (CC) nucleotide-binding (NB) leucine-rich repeat (LRR)-class resistance protein that confers resistance to a yellow strain of Cucumber mosaic virus [CMV(Y)] with developing the hypersensitive response (HR) cell death in virus-inoculated leaves of Arabidopsis thaliana. To understand the RCY1-mediated virus defense response, host protein that interacts with the CC and NB domains of RCY1 was isolated in a yeast two-hybrid system using the CC-NB domain fragment as bait. The WRKY70 transcription factor could reproducibly bind to the CC-NB domain fragment. This interaction between WRKY70 and the CC-NB domains was confirmed in a co-immunopurification experiment, suggesting that WRKY70 may function in RCY1-conferred resistance to CMV(Y). In CMV(Y)-inoculated leaves of wild-type plants carrying RCY1, virus multiplication was suppressed, and the level of WRKY70 and Pathogenesis-Related 1 (PR-I) transcripts clearly increased. On the other hand, in CMV(Y)-inoculated leaves of the RCY1-transformed wrky70-1 mutant lacking WRKY70 transcripts, virus coat protein accumulation increased significantly, and the induction of PR-1 expression in response to CMV(Y) was partially compromised. However, there was no significant difference in the degree of HR cell death between the wrky70-1 and wild-type plants carrying RCY1. These results indicate that WRKY70 may play a critical role in suppressing virus multiplication, but not in elaborating HR cell death, by directly interacting with the CC-NB domains of RCY1. (C) 2013 Elsevier Ltd. All rights reserved.