HUMAN IGE RESPONSES TO SCHISTOSOMA-MANSONI AND RESISTANCE TO REINFECTION
HUMAN IGE RESPONSES TO SCHISTOSOMA-MANSONI AND RESISTANCE TO REINFECTION
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DOI：
10.1590/s0074-02761992000800014
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发表时间：
1992-01-01
影响因子：
2.8
通讯作者：
STURROCK, RF
中科院分区：
文献类型：
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作者：
DUNNE, DW;BUTTERWORTH, AE;STURROCK, RF
曼氏血吸虫感染的肯尼亚患者接受了治疗，并在接下来的两年中对其再感染的强度进行了随访。此外，在ELISA中测定了其治疗前和治疗后6个月的血清IgG 1 -4、IgM和IgE水平，这些水平对童虫、虫卵和成虫具有特异性。治疗后6个月内未发生再感染。再感染强度随年龄而变化;年幼的儿童再次感染的强度明显高于年长者。当抗体和再感染水平进行了比较，只有6个月后治疗IgE response对成虫的强度呈负相关的再感染，因此，是抵抗或免疫再感染的预测。进行IgE和IgG特异性蛋白质印迹。成虫抗原识别的IgE相比，相同的患者的IgG反应是有限的，虽然没有个别抗原是唯一的识别IgE同种型。一个占主导地位的22 kDa抗原被大多数但不是所有的高IgE应答者所识别。与无应答者相比，对这种抗原有IgE应答的患者随后再感染的水平显著降低。单特异性兔抗血清对22 kDa的成虫抗原表明，这种抗原是专门位于皮层的成虫和“肺”和“肝”阶段的童虫，但不存在于早期的“皮肤”童虫。该抗原可能是人IgE介导的免疫效应机制的靶标，其对后皮肤期童虫具有活性，并且这通过蠕虫的死亡而增强。
Schistosoma mansoni infected Kenyan patients were treated and the intensities of their reinfections were followed over the next two years. In addition, their pre- and six month post-treatment serum levels of IgG1-4, IgM, and IgE, specific for schistosomula, egg and adult worm, were measured in ELISA. No reinfection took place before six months post-treatment. Reinfection intensities varied with age; the younger children becoming reinfected at significantly higher intensities than older individuals. When antibody and reinfection levels were compared, only the six month post-treatment IgE reponse against adult worm correlated negatively with intensities of reinfection and, therefore, was predictive of resistance or immunity to reinfection. IgE and IgG specific Western Blots were carried out. The adult worm antigens recognized by IgE were restricted compared with the IgG responses of the same patients, although no individual antigen was uniquely recognized by the IgE isotype. A dominant 22 kDa antigen was recognized by most but not all high IgE responders. Patients with IgE responses against this antigen suffered significantly lower subsequent levels of reinfection, compared with non-responders. A monospecific rabbit antiserum against the 22 kDa adult worm antigen showed that this antigen is specifically located in the tegument of the adult worm and of 'lung' and 'liver' stage schistosomula, but is absent from the early 'skin' schistosomula. It is possible that this antigen is a target for human IgE mediated immune effector mechanisms active against the post skin stage schistosomula and that this is boosted by the death of adult worms.