The CD3γ chain is essential for development of both the TCRαβ and TCRγδ lineages
The CD3γ chain is essential for development of both the TCRαβ and TCRγδ lineages
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DOI：
10.1093/emboj/17.7.1871
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发表时间：
1998-04-01
期刊：
影响因子：
11.4
通讯作者：
Kruisbeek, AM
中科院分区：
文献类型：
--
作者：
Haks, MC;Krimpenfort, P;Kruisbeek, AM
CD 3 γ和CD 3 δ是最密切相关的CD 3组分，两者都参与成熟T细胞上表达的TCR α β-CD 3复合物。然而，有趣的是，CD 3 δ似乎并没有在功能上参与在未成熟T细胞上表达的前T细胞受体（TCR）复合物：CD 3 δ基因表达的破坏对由前TCR控制的发育步骤没有影响。在这里，我们报告与CD 3 δ相反，CD 3 γ是前TCR的重要组成部分。我们产生了选择性缺乏CD 3 γ表达的小鼠，其中CD 3 δ、CD 3 γ、CD 3 ζ、pT α和TCR β的表达保持不受干扰。因此，除了CD 3 γ之外，用于组成前TCR的所有组分都是可用的。然而，在CD 3 γ缺陷小鼠中，T细胞发育受到严重抑制，胸腺中的细胞数量减少至
CD3 gamma and CD3 delta are the most closely related CD3 components, both of which participate in the TCR alpha beta-CD3 complex expressed on mature T cells. Interestingly, however, CD3 delta does not appear to participate functionally in the pre-T-cell receptor (TCR) complex that is expressed on immature T cells: disruption of CD3 delta gene expression has no effect on the developmental steps controlled by the pre-TCR, Here we report that in contrast with CD3 delta, CD3 gamma is an essential component of the pre-TCR. We generated mice selectively lacking expression of CD3 gamma, in which expression of CD3 delta, CD3 epsilon, CD3 zeta, pT alpha and TCR beta remained undisturbed. Thus, all components for composing a pre-TCR are available, with the exception of CD3 gamma. Nevertheless, T-cell development is severely inhibited in CD3 gamma-deficient mice, The number of cells in the thymus is reduced to