Membrane proteins PmpG and PmpH are major constituents of Chlamydia trachomatis L2 outer membrane complex
Membrane proteins PmpG and PmpH are major constituents of Chlamydia trachomatis L2 outer membrane complex
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DOI：
10.1111/j.1574-6968.2000.tb09098.x
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发表时间：
2000-05-15
影响因子：
2.1
通讯作者：
Birkelund, S
中科院分区：
文献类型：
--
作者：
Mygind, PH;Christiansen, G;Birkelund, S
衣原体的外膜复合体参与宿主细胞对衣原体的初始粘附和摄取。为了鉴定沙眼衣原体L2外膜中的新蛋白，采用十二烷基硫酸钠聚丙烯酰胺凝胶电泳分离蛋白。通过蛋白质谱的银染色，检测到100-110 kDa的主要蛋白质双联体。凝胶内胰蛋白酶消化和基质辅助激光解吸/电离质谱鉴定这些蛋白质为推定的外膜蛋白PmpG和PmpH。(C)2000年，欧洲微生物学会联合会。由Elsevier Science B. V.出版，版权所有。
The outer membrane complex of Chlamydia is involved in the initial adherence and ingestion of Chlamydia by the host cell. In order to identify novel proteins in the outer membrane of Chlamydia trachomatis L2, proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis. By silver staining of the protein profile, a major protein doublet of 100-110 kDa was detected. In-gel tryptic digestion and matrix-assisted laser desorption/ionization mass spectrometry identified these proteins as the putative outer membrane proteins PmpG and PmpH. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.