Temporal effects of human chorionic gonadotropin on expression of the circadian genes and steroidogenesis-related genes in human luteinized granulosa cells
Temporal effects of human chorionic gonadotropin on expression of the circadian genes and steroidogenesis-related genes in human luteinized granulosa cells
复制标题
人绒毛膜促性腺激素对人黄素化颗粒细胞昼夜节律基因和类固醇生成相关基因表达的时间影响
DOI：
10.1080/09513590.2017.1296423
复制
发表时间：
2017-01-01
影响因子：
2
通讯作者：
Zhou, Canquan
中科院分区：
文献类型：
--
作者：
Chen, Minghui;Xu, Yanwen;Zhou, Canquan
摘要目的：动物模型研究表明，黄体内存在生物钟，对维持妊娠至关重要。然而，黄体中是否存在生物钟及其与卵巢类固醇激素生成的关系尚不清楚。研究设计：从接受体外受精治疗的患者中纯化并体外培养人黄素化颗粒细胞。应用定量PCR技术，观察了人绒毛膜促性腺激素（hCG）处理后48 h内，黄体化颗粒细胞中昼夜节律基因和类固醇生成相关基因mRNA的积累模式。结果：我们发现，昼夜节律基因CLOCK，PER 2和BMAL 1在培养的人黄体化颗粒细胞中表达。在这些基因中，只有PER 2的表达显示振荡模式与16小时的周期后，这些细胞由hCG刺激。CLOCK和BMAL 1的表达没有显示出显著的振荡模式。类固醇急性调节蛋白（星星）基因的表达显示出类似于PER 2的振荡模式。hCG刺激后，CYP 11 A1、HSD 3B 2和CYP 19 A1的表达显著增加;然而，这些基因均未显示出显著的振荡模式。结论：人卵巢黄素化颗粒细胞中存在分子生物钟，可能与卵巢激素合成有关。
Abstract Objective: It has been shown in animal models that circadian clock exists in corpora luteum which is essential for maintaining pregnancy. However, it is unknown whether circadian clock exists in corpora luteum and its relation with steroidogenesis in human ovary. Study design: Human luteinized granulosa cells from patients who underwent in vitro fertilization treatment were purified and cultured in vitro. Accumulation patterns of circadian gene and steroidogenesis-related gene mRNAs in human luteinized granulosa cells were observed during the 48 hours after treatment with human chorionic gonadotropin (hCG) by quantitative PCR. Results: We found that the circadian genes CLOCK, PER2, and BMAL1 were expressed in cultured human luteinized granulosa cells. Among these genes, only expression of PER2 displayed oscillating patterns with a 16-h period in these cells after stimulation by hCG. Expression of CLOCK and BMAL1 did not show significant oscillating patterns. Expression of the steroidal acute regulatory protein (STAR) gene showed an oscillating pattern that was similar to that of PER2. Expression of CYP11A1, HSD3B2, and CYP19A1 increased significantly after hCG stimulation; however, none of these genes displayed significant oscillating patterns. Conclusions: Molecular circadian clock exists in human luteinized granulosa cells and may be related with steroidogenesis in human ovary.