A nuclear cAMP binding protein in retinoic acid-treated HL-60 cells.
A nuclear cAMP binding protein in retinoic acid-treated HL-60 cells.
复制标题
视黄酸处理的 HL-60 细胞中的核 cAMP 结合蛋白。
DOI：
10.1002/jcp.1041360127
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发表时间：
1988
影响因子：
5.6
通讯作者：
Casey,SB
中科院分区：
文献类型：
--
作者：
Briggs,RC;Casey,SB
使用8-叠氮基[32 P]cAMP的光亲和标记在HL-60细胞中检测到cAMP结合蛋白。在未处理HL-60细胞和用视黄酸诱导成熟的HL-60细胞的0.35 M NaCl核蛋白提取物中发现了结合蛋白。虽然cAMP结合蛋白的量在诱导分化后没有变化，但在维甲酸处理后3天和5天存在第二种形式的亚基，改变了电荷。研究结果表明，II型cAMP依赖性蛋白激酶的调节亚基可能参与与人髓系细胞分化相关的核功能。
A cAMP binding protein was detected in HL‐60 cells using photoaffinity labeling with 8‐azido [32P]cAMP. The binding protein was found in a 0.35 M NaCl nuclear protein extract from untreated HL‐60 cells and from the HL‐60 cells induced to mature with retinoic acid. While the quantity of the cAMP binding protein did not change following the induced differentiation, a second form of the subunit, altered in charge, was present at 3 and 5 days after retinoic acid treatment. The findings indicate that the regulatory subunit of the type II cAMP‐dependent protein kinase could be involved in nuclear functions associated with human myeloid cell differentiation.