ACTION OF ADH ON ISOLATED MEDULLARY THICK ASCENDING LIMB OF THE BRATTLEBORO RAT
ACTION OF ADH ON ISOLATED MEDULLARY THICK ASCENDING LIMB OF THE BRATTLEBORO RAT
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DOI：
10.1152/ajprenal.1986.251.2.f271
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发表时间：
1986-08-01
影响因子：
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通讯作者：
STONER, L
中科院分区：
文献类型：
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作者：
BESSEGHIR, K;TRIMBLE, ME;STONER, L
在Brattleboro（尿崩症，DI）大鼠长期给予精氨酸加压素（抗利尿激素，ADH）期间建立最大皮质-髓质渗透压梯度是一个渐进的过程。在离体灌注的DI大鼠延髓粗升支（mTAL）上研究了ADH对电压和放射性同位素氯流出（管腔至浴）的影响。mTAL在体外急性暴露于ADH（250 μ U/ml）显著地增加了电压（+3.3 . ±. 0.3至+4.5 .+-。0.5 mV）和氯离子流出（192.7 . ±. 29.4至240.4 .+-。41.5 peq/min. cntdot. mm）。在用ADH慢性体内处理10-21天后，mTAL表达显著更高的基础电压和氯离子流出（+8.4 . 0.6 mV和393.2 ±. 71.6 peq/min. cntdot. mm）。在体外将ADH急性应用于长期治疗动物的mTAL诱导电压进一步小幅增加（22%）。这些结果表明，ADH可能有双重影响的NaCl运输的mTAL的DI大鼠：一个小的快速效应，和一个更大的长期增加运输，可以显示只有在慢性给药ADH。这些影响可能，部分地解释了在DI大鼠中观察到的集中能力的逐渐增强。
Establishment of a maximal corticomedullary osmotic gradient during chronic administration of arginine vasopressin (antidiuretic hormone, ADH) to Brattleboro (diabetes insipidus, DI) rats is a gradual process. The effects of ADH on voltage and radioisotopic chloride efflux (lumen to bath) were investigated in medullary thick ascending limb (mTAL) isolated from DI rats and perfused in vitro. Acute in vitro exposure of mTAL to ADH (250 .mu.U/ml) significantly increased both the voltage (+3.3 .+-. 0.3 to +4.5 .+-. 0.5 mV) and chloride efflux (192.7 .+-. 29.4 to 240.4 .+-. 41.5 peq/min .cntdot. mm). After chronic in vivo treatment with ADH for 10-21 days mTAL expressed substantially higher basal voltage and chloride efflux (+8.4 .+-. 0.6 mV and 393.2 .+-. 71.6 peq/min .cntdot. mm). Acute in vitro application of ADH to mTAL from chronically treated animals induced a further small increase in voltage (22%). These results are taken to indicate that ADH may have dual effects on NaCl transport by the mTAL of the DI rat: a small rapid effect, and a larger long-term increase in transport that can be shown only after chronic administration of ADH. These effects may, in part, explain the gradual enhancement of concentrating ability observed in DI rats.