Topology of membrane proteins
Topology of membrane proteins
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DOI：
10.1021/ci0001280
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发表时间：
2001-03-01
期刊：
影响因子：
--
通讯作者：
Simon, I
中科院分区：
文献类型：
--
作者：
Tusnády, GE;Simon, I
整合膜蛋白在活细胞中起着重要作用。由于实验技术的困难，理论方法，即，拓扑预测方法对于这类蛋白质的结构确定是重要的。在这里，我们展示了跨膜拓扑预测方法的详细比较。根据这种比较，我们得出结论，完整的膜蛋白的拓扑结构是由序列片段的氨基酸组成的最大分歧。这些片段位于细胞的不同区域，其特征在于不同的物理化学性质。这些预测方法的结果相比，几个跨膜蛋白的X-射线衍射数据也将进行讨论。
Integral membrane proteins play important roles in living cells. Due to difficulties of experimental techniques, theoretical approaches, i.e., topology prediction methods, are important for structure determination of this class of proteins. Here we show a detailed comparison of transmembrane topology prediction methods. According to this comparison, we conclude that the topology of integral membrane proteins is determined by the maximum divergence of the amino acid composition of sequence segments. These segments are located in different areas of the cell, which can be characterized by different physicochemical properties. The results of these prediction methods compared to the X-ray diffraction data of several transmembrane proteins will also be discussed.