Impact of mixed biofilm formation with environmental microorganisms on E. coli O157:H7 survival against sanitization.
Impact of mixed biofilm formation with environmental microorganisms on E. coli O157:H7 survival against sanitization.
复制标题
与环境微生物的混合生物膜形成对大肠杆菌O157：H7生存的影响。
DOI：
10.1038/s41538-020-00076-x
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发表时间：
2020
影响因子：
6.4
通讯作者：
Wang R
中科院分区：
文献类型：
--
作者：
Chitlapilly Dass S;Bosilevac JM;Weinroth M;Elowsky CG;Zhou Y;Anandappa A;Wang R
食源性病原体形成生物膜是对食品安全和公众健康的严重威胁。肉类加工厂可能有各种微生物和偶尔的食源性病原体；因此，环境微生物群落可能通过混合生物膜的形成影响病原体的生存。本研究收集了两家具有不同O157:H7大肠杆菌流行史的牛肉工厂的地沟样本，研究了环境微生物对病原菌消毒剂耐受性的影响。结果表明，不同植物和排水口位置的生物膜形成能力和细菌种类组成差异较大。大肠杆菌O157:H7在与复发性大肠杆菌O157:H7流行的植物样本混合的生物膜中获得的消毒剂耐受性明显高于与其他植物样本混合的生物膜。混合生物膜对大肠杆菌O157:H7的保护效果最好，物种多样性也最高。消毒处理后的物种百分比发生了显著变化，表明群落组成影响了每个物种的作用和耐受水平。因此，独特的环境微生物群落，它们在接触表面形成生物膜的能力和种间相互作用都通过增强或减少生物膜群落内病原体的存活，在大肠杆菌O157:H7的持久性中发挥作用。
Biofilm formation by foodborne pathogens is a serious threat to food safety and public health. Meat processing plants may harbor various microorganisms and occasional foodborne pathogens; thus, the environmental microbial community might impact pathogen survival via mixed biofilm formation. We collected floor drain samples from two beef plants with different E. coli O157:H7 prevalence history and investigated the effects of the environmental microorganisms on pathogen sanitizer tolerance. The results showed that biofilm forming ability and bacterial species composition varied considerably based on the plants and drain locations. E. coli O157:H7 cells obtained significantly higher sanitizer tolerance in mixed biofilms by samples from the plant with recurrent E. coli O157:H7 prevalence than those mixed with samples from the other plant. The mixed biofilm that best protected E. coli O157:H7 also had the highest species diversity. The percentages of the species were altered significantly after sanitization, suggesting that the community composition affects the role and tolerance level of each individual species. Therefore, the unique environmental microbial community, their ability to form biofilms on contact surfaces and the interspecies interactions all play roles in E. coli O157:H7 persistence by either enhancing or reducing pathogen survival within the biofilm community.
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影响因子：
4.2
作者：
Das JK;Mishra D;Ray P;Tripathy P;Beuria TK;Singh N;Suar M
通讯作者：
Suar M
影响因子：
2
作者：
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作者：
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2
作者：
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通讯作者：
Arthur, Terrance M.
影响因子：
2
作者：
Wang, Rong;Kalchayanand, Norasak;Arthur, Terrance M.
通讯作者：
Arthur, Terrance M.