Chitosan crosslinked with genipin as supporting matrix for biodegradation of synthetic dyes: Laccase immobilization and characterization
Chitosan crosslinked with genipin as supporting matrix for biodegradation of synthetic dyes: Laccase immobilization and characterization
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壳聚糖与京尼平交联作为合成染料生物降解的支持基质：漆酶固定和表征
DOI：
10.1016/j.cherd.2018.02.008
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发表时间：
2018-04-01
影响因子：
3.9
通讯作者：
Dai, Yu-Cheng
中科院分区：
文献类型：
--
作者：
Ma, Hong-Fei;Meng, Ge;Dai, Yu-Cheng
漆酶具有节能环保的特点，可作为绿色催化剂应用于工业领域。通过将白腐菌Trametes pubescens的漆酶包埋在交联剂Genipin接枝的壳聚糖珠中，设计了一种高效、稳定的降解合成染料的生物催化剂。以0.10%(v/v)的京尼平和4.0U m L(-1)的酶溶液分别活化14h和6h的固定化效果最好。与游离漆酶相比，固定化漆酶的pH、热稳定性和贮存稳定性都得到了提高。壳聚糖漆酶体系在11个循环后的残存活力为>；55%，表现出比游离漆酶更好的耐久性，这反映了京尼平作为交联剂用于漆酶固定化的可行性。游离漆酶在4℃下存放30天后，酶活损失为84.43%，而壳聚糖漆酶体系在此期间的酶活损失为42.86%。仅固定化漆酶就能更有效地脱色结构不同的合成染料，而不需要氧化介质来发挥其生物降解能力。这些发现表明，通过将真菌漆酶包埋在京尼平活化的壳聚糖珠中，可以提高该酶的稳定性和降解能力。(C)2018年化学工程师学会。爱思唯尔出版，版权所有。
Being energy-saving and environmentally friendly, laccases could be introduced as green catalysts for industrial applications. A highly stable and efficient biocatalyst for degrading synthetic dyes was designed by entrapping a purified laccase from the white rot fungus Trametes pubescens within chitosan beads grafted by the cross-linker genipin. Maximum immobilization was obtained with 0.10% (v/v) genipin and 4.0 U mL(-1) of enzyme solution after activation and contact for 14h and 6h, respectively. Improved pH, thermal, and storage stabilities of the immobilized laccase were obtained when compared with the free counterpart. The chitosan laccase system exhibited a residual activity of >55% after 11 cycles, demonstrating better durability than the free laccase, which reflected the suitability of genipin as a cross-linker for laccase immobilization. The activity loss of free laccase after 30 days of storage at 4 degrees C was 84.43%, whereas the chitosan laccase system had a loss of 42.86% during the same period. The immobilized laccase alone more efficiently decolorized structurally different synthetic dyes without requiring oxidizing mediators to function in its biodegradation capability. These findings are indicative that the stability and biodegradation capability of fungal laccase can be enhanced by entrapping the enzyme within genipin-activated chitosan beads. (C) 2018 Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.