Assessment of incising ethology in the absence and presence of jaw muscle hyperalgesia in a mouse home cage environment.
Assessment of incising ethology in the absence and presence of jaw muscle hyperalgesia in a mouse home cage environment.
复制标题
在小鼠家笼环境中评估是否存在下颌肌肉痛觉过敏的切割行为学。
DOI：
10.1016/j.physbeh.2015.06.015
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发表时间：
2015
影响因子：
2.9
通讯作者：
Morris-Wiman,J
中科院分区：
文献类型：
--
作者：
Widmer,CG;Morris-Wiman,J
由于缺乏目前可用的非侵入性和允许在家庭笼子环境中进行长期评估的技术，对小鼠口腔运动行为的评估具有挑战性。本研究的目的是利用安装在现有家养笼子上的三维力传感器来评估小鼠的切入行为。此外，一个持续的痛觉过敏条件被引入来评估该技术的敏感性，以识别切口行为的变化。方法采用自定义编写的软件，对CD-1雄性和雌性小鼠（n = 48）在4次基线和6次纵向痛觉过敏评估期间的24小时记录时间内的神经元活性进行评估。采用临床前持续性疼痛模型，反复注射生理盐水诱导咬肌痛觉过敏。在基线和痛觉过敏条件下的光照和黑暗周期中，性别和年龄差异对多个切口变量进行了评估。结果不同性别间存在显著性差异，但年龄间无显著性差异。在4.6-10.4 Hz范围内确定了离散的切割频率，并且在雌性和雄性小鼠中均可重复发现。与中性生理盐水注射相比，重复注射酸性生理盐水后观察到明显的低切割频率的转变。大约四周后，这种向低频率切割的转变又回到了基线水平，但从统计学上讲，雌性小鼠比雄性小鼠的时间更长。在痛觉过敏状态下，食物摄取量（减少）和体重变化也有显著差异。两组在总切口数、每日切口次数和切口力方面均无显著差异。结论本研究的结果支持使用记录三维切牙力作为切牙行为的敏感测量。这种新技术允许识别特定的切口变量，这些变量在雌性和雄性小鼠持续痛觉过敏期间受到不同的影响。数据是在家庭笼环境中收集的，最小的偏差，如实验者的互动。与其他牙痛研究类似，即使在痛觉过敏的情况下，小鼠也能够保持每天正常的切口活动水平（切口总数，切口发作总数）。
IntroductionAssessment of oral motor behavior in a mouse is challenging due to the lack of currently available techniques that are non-invasive and allow long-term assessment in a home cage environment. The purpose of this study was to evaluate incising behavior using mouse chow attached to a three-dimensional force transducer that was mounted on the existing home cage. In addition, a persistent hyperalgesia condition was introduced to evaluate the sensitivity of the technique to identify incising behavioral changes.MethodsIncising activity of CD-1 male and female mice (n = 48) was evaluated over a 24 hour recording session during four baseline and six longitudinal hyperalgesia assessment sessions using custom written software. A pre-clinical persistent pain model was used to induce hyperalgesia in the masseter muscle by repetitive acidic saline injections. Sex and age differences were evaluated for multiple incising variables during both light and dark cycles during baseline and hyperalgesia conditions.ResultsSignificant sex differences were found for multiple incising variables but not for age. Discrete incising frequencies were identified in the range of 4.6–10.4 Hz and were reproducibly found in both female and male mice. A significant shift to lower incising frequencies was observed after repetitive acidic saline injections compared to neutral saline injections. This shift to lower frequencies of incising returned to baseline levels after approximately four weeks but was statistically longer in female compared to male mice. Significant differences were also found for chow intake (reduced) and weight change during the hyperalgesia condition. No significant differences were found for total number of incisions or number of incising episodes per day or incising force.ConclusionsThe findings from this study support the use of recording three dimensional incising forces as a sensitive measure of incising behavior. This novel technique allowed the identification of specific incising variables that were differentially affected in female and male mice during a persistent hyperalgesia. The data were collected in the home cage environment with minimal bias such as experimenter interaction. Similar to other dental pain studies, mice were able to maintain normal incising activity levels per day (total incisions, total number of incising episodes) even in the presence of hyperalgesia.