α-Lactalbumin Self-Assembled Nanoparticles with Various Morphologies, Stiffnesses, and Sizes as Pickering Stabilizers for Oil-in-Water Emulsions and Delivery of Curcumin
α-Lactalbumin Self-Assembled Nanoparticles with Various Morphologies, Stiffnesses, and Sizes as Pickering Stabilizers for Oil-in-Water Emulsions and Delivery of Curcumin
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DOI：
10.1021/acs.jafc.0c06263
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发表时间：
2021-02-08
影响因子：
6.1
通讯作者：
Li, Yuan
中科院分区：
文献类型：
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作者：
Liu, Bin;Liu, Bingxue;Li, Yuan
为了系统研究纳米颗粒（NPs）对皮克林乳状液（PEs）的稳定性、界面活性和消化性能的多重影响，以不同形态、刚度和大小的自组装α -乳清蛋白衍生肽为基础，用NPs制备了多种水包油（pe）。我们发现，与Turbiscan观察到的其他纳米颗粒相比，由小尺寸和柔软的纳米球（NSs）稳定的PEs在储存过程中具有最高的稳定性。膨胀界面流变性分析表明，NSs在油水界面有序堆积形成了高弹性的界面膜。同时，NSs稳定的皮克林乳最稳定，脂质消化率最低。管状NPs在油水界面分布不均匀，界面活性较低。较硬的NPs柔韧性较低，乳状液稳定性较差。在pe中加入姜黄素，进一步研究其对生物利用度的影响。体内药动学结果表明，经NSs稳定的皮克林乳剂具有最高的姜黄素生物利用度，比未包封的姜黄素高5.37倍。研究表明，稳定性最佳的NSs稳定的皮克林乳状液是最有前途的疏水生物活性成分递送体系。
To systematically study the multiple effects of nanoparticles (NPs) on the stability, interfacial activity, and digestive properties of Pickering emulsions (PEs), various oil-in-water PEs were prepared by NPs based on the self-assembled alpha-lactalbumin-derived peptides with a variety of morphologies, stiffnesses, and sizes. We discovered that PEs stabilized by small-sized and soft nanospheres (NSs) exhibited the highest stability compared with other nanoparticles observed by Turbiscan during storage. Dilational interfacial rheological analysis demonstrated that a highly elastic interfacial film of the NSs had been formed by orderly packing at oil/water interfaces. Meanwhile, the most stable Pickering emulsion stabilized by NSs possessed the lowest lipid digestion rate. The tubular NPs distributed unevenly at the oil-water interfaces therefore showed lower interfacial activity. Harder NPs with lower flexibility showed a lower emulsion stability. Curcumin was loaded in PEs to further study the effect of bioavailability. Moreover, in vivo pharmacokinetic results revealed that Pickering emulsion stabilized by NSs showed the highest curcumin bioavailability, which was 5.37 times higher than unencapsulated curcumin. This study suggested that Pickering emulsion stabilized by NSs with the optimum stability was the most promising delivery system for hydrophobic bioactive ingredients.