Proliferation in human gastrointestinal epithelium using bromodeoxyuridine in vivo: data for different sites, proximity to a tumour, and polyposis coli.
Proliferation in human gastrointestinal epithelium using bromodeoxyuridine in vivo: data for different sites, proximity to a tumour, and polyposis coli.
复制标题
体内使用溴脱氧尿苷在人胃肠道上皮中的增殖：不同部位、肿瘤附近和结肠息肉病的数据。
作者：
Christopher S. Potten;M. Kellett;David A. Rew;Stephen A Roberts
本文用免疫组织化学方法研究了病人注射溴脱氧尿苷后，DNA合成细胞在人小肠和大肠上皮细胞隐窝中的分布与细胞在隐窝中的位置的关系。对正常上皮的不同部位进行了研究。回肠具有较短的隐窝和非常显著的较小的细胞总数。然而，它的峰值标记指数(LI)值与结肠相似，而直肠的峰值LI值较低。标记的平均位置在回肠的第17个细胞位置，在结肠和直肠中大约在第22个位置。总体平均LI在回肠较高，为17.8%，结肠居中，为10.3%，直肠最低，为8.5%。因此，发生肿瘤的可能性和LI测量的细胞增殖率之间存在着相反的关系。假设S的持续时间为8.6h，数据表明，回肠和结肠中隐窝区域的细胞周期时间约为30小时，直肠约为37小时。在肿瘤附近(1厘米以内)采集的样本显示，与距离肿瘤5厘米以上的样本相比，所有细胞位置的增殖活性普遍受到抑制。LI的平均值说明了这一点，在邻近肿瘤的结肠中，LI约为5.4%，而在距离肿瘤较远的结肠中，LI约为10%；直肠的可比值分别为4.6%和8.5%。取自两名结肠息肉病患者的样本显示，与正常结肠相比，分布的偏斜度有显著差异，分布普遍向右移动，即细胞位置较高。在息肉病结肠标本中，平均细胞位置和LI峰的位置显著增加。
The distribution of DNA synthesising cells in the crypts of the epithelium in human small and large bowel after injection of bromodeoxyuridine into patients has been studied in relation to the position of the cells in the crypt using immunohistochemistry. Different sites of normal epithelium have been studied. The ileum has a shorter crypt and a very significantly smaller total cell population size. However, it has similar peak labelling index (LI) values to the colon, while the rectum has a lower peak LI value. The mean position of the label occurs at the 17th cell position in the ileum and at about the 22nd position in both the colon and rectum. The overall mean LI is significantly higher in the ileum at 17.8%, intermediate in the colon at 10.3%, and lowest in the rectum at 8.5%. There is thus an inverse relation between the likelihood of developing a tumour and the rate of cell proliferation as measured by the LI. Assuming a value of 8.6 hours for the duration of S, the data suggest that the cell cycle time in the mid crypt region is about 30 hours for the ileum and colon and about 37 hours for the rectum. Samples taken adjacent (within 1 cm) to a tumour show a general dampening of proliferative activity at all cell positions compared with samples taken more than 5 cm from a tumour. This is illustrated by the average LI, which is about 5.4% in the colon adjacent to a tumour compared with 10% distant; comparable values for the rectum are 4.6% and 8.5%. Samples taken from two patients with polyposis coli show distributions with a significant difference in skewness compared with normal colon and a general shifting of the distribution to the right, that is to higher cell positions. There is a significant increase in the mean cell position and the position of the peak LI in the polyposis coli samples.