Diversity and succession of autotrophic microbial community in high-elevation soils along deglaciation chronosequence.
Diversity and succession of autotrophic microbial community in high-elevation soils along deglaciation chronosequence.
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DOI：
10.1093/femsec/fiw160
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发表时间：
2016-10
影响因子：
4.2
通讯作者：
Jinbo Liu;W. Kong;Guo-shuai Zhang;Ajmal Khan;G. Guo;Chunmao Zhu;Xiaojie Wei;Shi-chang Kang
中科院分区：
文献类型：
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作者：
Jinbo Liu;W. Kong;Guo-shuai Zhang;Ajmal Khan;G. Guo;Chunmao Zhu;Xiaojie Wei;Shi-chang Kang
全球变暖导致高海拔地区的冰川大量退缩，使冰川消退的土壤暴露在恶劣的环境条件下。自养微生物是冰川消退土壤中的先驱定居者，为缺乏植被的极端生态系统提供营养。然而，在冰川消退的土壤中，自养群落的研究仍然较少。我们探讨了cbbL基因编码大亚基的I型RubisCO，一个关键的CO2固定酶，利用分子生物学方法在冰川消退的土壤沿着10年的冰川消退时序在青藏高原的多样性和继承。结果表明，在幼龄土壤（0-2.5年生）中，I型cbbL（IA/B、IC和ID）的丰度迅速增加，而在老龄土壤中保持稳定。土壤总有机碳（TOC）和总氮（TN）含量沿时间序列沿着逐渐增加，并与细菌和自养生物的丰度呈正相关，表明土壤TOC和TN来源于自养生物。与蓝藻相关的IA/B型自养菌的丰度明显高于IC和ID型。蓝藻多样性和均匀性在幼龄土壤（<6年生）中增加，然后保持稳定。我们的研究结果表明，蓝细菌起着重要的作用，在积累TOC和总氮在冰川消退的土壤。
Global warming has resulted in substantial glacier retreats in high-elevation areas, exposing deglaciated soils to harsh environmental conditions. Autotrophic microbes are pioneering colonizers in the deglaciated soils and provide nutrients to the extreme ecosystem devoid of vegetation. However, autotrophic communities remain less studied in deglaciated soils. We explored the diversity and succession of the cbbL gene encoding the large subunit of form I RubisCO, a key CO2-fixing enzyme, using molecular methods in deglaciated soils along a 10-year deglaciation chronosequence on the Tibetan Plateau. Our results demonstrated that the abundance of all types of form I cbbL (IA/B, IC and ID) rapidly increased in young soils (0-2.5 years old) and kept stable in old soils. Soil total organic carbon (TOC) and total nitrogen (TN) gradually increased along the chronosequence and both demonstrated positive correlations with the abundance of bacteria and autotrophs, indicating that soil TOC and TN originated from autotrophs. Form IA/B autotrophs, affiliated with cyanobacteria, exhibited a substantially higher abundance than IC and ID. Cyanobacterial diversity and evenness increased in young soils (<6 years old) and then remained stable. Our findings suggest that cyabobacteria play an important role in accumulating TOC and TN in the deglaciated soils.