Microarray-based analysis of changes in diversity of microbial genes involved in organic carbon decomposition following land use/cover changes.
Microarray-based analysis of changes in diversity of microbial genes involved in organic carbon decomposition following land use/cover changes.
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DOI：
10.1111/j.1574-6968.2006.00511.x
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发表时间：
2007
影响因子：
2.1
通讯作者：
Yuguang Zhang;Xiaoquan Zhang;Xue-duan Liu;Ye Xiao;Liangjian Qu;Liyou Wu;Jizhong Zhou
中科院分区：
文献类型：
--
作者：
Yuguang Zhang;Xiaoquan Zhang;Xue-duan Liu;Ye Xiao;Liangjian Qu;Liyou Wu;Jizhong Zhou
为了进一步了解土地利用/覆被变化对土壤微生物分解基因的影响，分析了四川西部亚高山地区4个不同土地利用/覆被历史的土壤样品。第一个地点是岷江冷杉原始林，第二个和第三个地点分别是20世纪60年代和80年代建立的云杉人工林，第四个地点是20世纪60年代建立的农田。分离微生物群落的基因组DNA，并与含有1，961个探针的功能基因微阵列杂交。原始林、云杉人工林和农田中分别有39、62、41和28个基因探针具有显著的阳性信号，基因多样性指数（H '）分别为3.59、4.04、3.70和3.16。结果表明，除原始岷江冷杉林外，功能基因的数量和基因多样性指数与土壤有机碳含量的增加相关。聚类分析表明，原始森林土壤与60年代云杉种植园土壤聚类更近。
To increase our understanding of the impact of land use/cover changes on soil microbial decomposition genes involved in organic carbon decomposition, we analyzed soil samples in four sites with different land cover/use histories in a subalpine region of western Sichuan. One site was in a primitive Abies faxoniana forest, the second and the third sites were spruce plantations established in 1960's and 1980's, respectively, and the fourth site was in a cropland dating back to 1960's. The genomic DNA from the microbial community was isolated and hybridized against a functional gene microarray containing 1,961 probes. There were 39, 62, 41, and 28 gene probes with statistically significant positive signals and the gene diversity index (H') values were 3.59, 4.04, 3.70 and 3.16 in primitive forest, spruce plantations established in 1960s and 1980s and cropland, respectively. The results suggested that the number of functional genes and the gene diversity index were correlated with increasing amounts of soil organic carbon, except in the primitive Abies faxoniana forest site. cluster analysis demonstrated that primitive forest soil was clustered more closely to soil from the spruce plantation established in 1960s.