The differential stimulation of brain and heart cyclic-AMP phosphodiesterase by oncomodulin.
The differential stimulation of brain and heart cyclic-AMP phosphodiesterase by oncomodulin.
复制标题
癌调节素对脑和心脏环磷酸二酯酶的差异刺激。
DOI：
10.1016/0006-291x(85)91830-3
复制
发表时间：
1985
影响因子：
3.1
通讯作者：
J. Macmanus
中科院分区：
文献类型：
--
作者：
B. Mutus;N. Karuppiah;R. Sharma;J. Macmanus
Ca2+/ calmodulin dependent cyclic nucleotide phosphodiesterase, from the bovine heart and brain, purified by monoclonal antibody chromatography were tested with respect to activation by oncomodulin. The heart and brain enzymes which have previously been shown to have slightly different electrophoretic mobilites (1), were found to also differ in the oncomodulin dose-dependent activation of cAMP hydrolysis. Oncomodulin was shown to activate the heart enzyme to the same extent as calmodulin. However, this study indicates that the heart phosphodiesterase has approximately 25-fold higher affinity for oncomodulin than the brain enzyme. The oncomodulin concentration required for the half-maximal activation of the heart phosphodiesterase was estimated to be 2 × 10−7M. In addition, the possibility of the observed activation by oncomodulin being due to calmodulin contamination can be ruled out as the oncomodulin activation profiles were unaltered subsequent to chromatography on organomercurial agarose and the activation by oncomodulin could not be reversed by anti-calmodulin IgG.
