Positional information in root epidermis is defined during embryogenesis and acts in domains with strict boundaries
Positional information in root epidermis is defined during embryogenesis and acts in domains with strict boundaries
复制标题
DOI：
10.1016/s0960-9822(98)70176-9
复制
发表时间：
1998-04-09
期刊：
影响因子：
9.2
通讯作者：
Dolan, L
中科院分区：
文献类型：
--
作者：
Berger, F;Haseloff, J;Dolan, L
背景：细胞位置而不是细胞谱系决定了植物发育的大部分方面。然而，位置信息的性质和起源仍然难以捉摸。在许多情况下，动物表皮的形成依赖于细胞间通讯所提供的位置信息。拟南芥根的表皮层是由两种细胞类型的交替文件，从而提出了一个简单的模式，以研究位置mechanism.Results：结合分子和形态标记的根表皮的克隆分析表明，细胞的命运是由相对于底层细胞层，皮层的位置。表皮图案似乎是在胚胎发生过程中组织起来的。然而，发育中的根的命运并不固定，因为细胞移动到先前被使用激光显微手术消融的相邻细胞占据的位置会改变命运。相反，当与活的相邻细胞的通信受损时，细胞命运不会改变。精确映射的位置的细胞外的线索对细胞命运的影响已经表明，域的位置信息组织良好定义的boundaries.Conclusions：细胞命运的规范在根表皮依赖于位置信息，组织在稳定的域与尖锐的边界。表皮图案在胚胎发生期间被定义，并且位置信息在根中保持活跃，直到细胞形态发生开始。一些位置线索的来源可能是细胞外的。(C)Current Biology Ltd ISSN 0960-9822。
Background: Cell position rather than cell lineage governs most aspects of development in plants. However, the nature and the origin of positional information remains elusive. Animal epidermal patterning relies in many cases on positional information provided by cell-cell communication. The epidermal layer of the Arabidopsis root is made of alternating files of two cell types and thus presents a simple pattern to study positional mechanisms.Results: Clonal analysis of the root epidermis in combination with molecular and morphological markers has shown that cell fate is determined by position relative to the underlying cell layer, the cortex. The epidermal pattern appears to be organised during embryogenesis. Fate is not fixed in the developing root, though, as cells that move into a position previously occupied by neighbour cells ablated using laser microsurgery change fate. In contrast, cell fate is not altered when communication with living neighbour cells is impaired. Precise mapping of the influence of the position of extracellular cues on cell fate has shown that domains of positional information are organised with well-defined boundaries.Conclusions: Cell-fate specification in the root epidermis relies on positional information that is organised in stable domains with sharp boundaries. The epidermal pattern is defined during embryogenesis and positional information remains active in the root until the initiation of cell morphogenesis. The origin of some positional cues might be extracellular. (C) Current Biology Ltd ISSN 0960-9822.