Absence of p53 gene mutations in primary nasopharyngeal carcinomas.
Absence of p53 gene mutations in primary nasopharyngeal carcinomas.
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发表时间：
1992-09
期刊：
影响因子：
11.2
通讯作者：
Charles H. Spruck;Y. Tsai;D. Huang;Allen S. Yang;William M. Rideout;M. Gonzalez‐Zulueta;Peter S. Choi;K. Lo;Mimi C. Yu;Peter A. Jones
中科院分区：
文献类型：
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作者：
Charles H. Spruck;Y. Tsai;D. Huang;Allen S. Yang;William M. Rideout;M. Gonzalez‐Zulueta;Peter S. Choi;K. Lo;Mimi C. Yu;Peter A. Jones
Alterations in the p53 tumor suppressor gene and Epstein-Barr virus status were investigated in 15 nasopharyngeal carcinoma (NPC) biopsies, 4 xenografts, and 2 cell lines from the Cantonese region of southern China. One other established NPC cell line obtained from a northern Chinese patient was also studied. Restriction fragment length polymorphism analysis revealed a loss of heterozygosity for chromosome 17p, where the p53 gene resides, in only one of 15 NPC biopsies. Polymerase chain reaction-single-stranded conformational polymorphism analysis and direct sequencing failed to detect sequence alterations in exons 5 through 8 of the p53 gene in the 15 tumors and in the 4 NPC xenografts, all of which tested positive for Epstein-Barr virus. In contrast, the 3 NPC cell lines were all negative for Epstein-Barr virus and contained G----C transversions in the p53 gene, with cell lines CNE-1 and CNE-2 harboring identical AGA (arginine) to ACA (threonine) changes at codon 280. These results suggest that p53 inactivation is not a necessary component of nasopharyngeal carcinogenesis in Cantonese but may be important in the establishment of cell lines derived from these tumors.
