Genomic expansion and clustering of ZAD-containing C2H2 zinc-finger genes in Drosophila
Genomic expansion and clustering of ZAD-containing C2H2 zinc-finger genes in Drosophila
复制标题
DOI：
10.1093/embo-reports/kvf243
复制
发表时间：
2002-12-01
期刊：
影响因子：
7.7
通讯作者：
Böhm, S
中科院分区：
文献类型：
--
作者：
Chung, HR;Schäfer, U;Böhm, S
C2 H2锌指蛋白是高等真核生物中最大的核酸结合因子家族。在果蝇基因组中，计算机分析共鉴定出326个假定的ZFP基因，相当于注释基因的2.3%。大约29%的果蝇ZFP在人类和/或秀丽隐杆线虫中是进化保守的。此外，约28%的ZFP含有由75个氨基酸残基组成的N端锌指相关C4DM结构域（ZAD）。ZAD仅限于双翅目和密切相关的昆虫的ZFP。进化的限制，扩大ZAD-含有ZFP基因在果蝇基因组和它们的聚类在几个染色体位点的功能让人想起脊椎动物KRAB-ZFP。ZAD可能代表蛋白质-蛋白质相互作用结构域。我们建议，ZAD含有ZFP基因直接或通过位点特异性修饰和/或染色质的调节参与转录调控。
C2H2 zinc-finger proteins (ZFPs) constitute the largest family of nucleic acid binding factors in higher eukaryotes. In silico analysis identified a total of 326 putative ZFP genes in the Drosophila genome, corresponding to similar to2.3% of the annotated genes. Approximately 29% of the Drosophila ZFPs are evolutionary conserved in humans and/or Caenorhabditis elegans. In addition, similar to28% of the ZFPs contain an N-terminal zinc-finger-associated C4DM domain (ZAD) consisting of similar to75 amino acid residues. The ZAD is restricted to ZFPs of dipteran and closely related insects. The evolutionary restriction, an expansion of ZAD-containing ZFP genes in the Drosophila genome and their clustering at few chromosomal sites are features reminiscent of vertebrate KRAB-ZFPs. ZADs are likely to represent protein-protein interaction domains. We propose that ZAD-containing ZFP genes participate in transcriptional regulation either directly or through site-specific modification and/or regulation of chromatin.