Expression and Functional Characterization of Retinoic Acid-Inducible Gene-I-Like Receptors of Mast Cells in Response to Viral Infection
Expression and Functional Characterization of Retinoic Acid-Inducible Gene-I-Like Receptors of Mast Cells in Response to Viral Infection
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DOI：
10.1159/000343895
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发表时间：
2013-01-01
影响因子：
5.3
通讯作者：
Ogawa, Hideoki
中科院分区：
文献类型：
--
作者：
Fukuda, Minoru;Ushio, Hiroko;Ogawa, Hideoki
为了研究肥大细胞识别病毒的确切机制，研究了导致肥大细胞活化的病毒识别受体的表达和功能特征。我们的研究结果表明，肥大细胞是部分负责早期在体内生产的抗病毒细胞因子和趋化因子后水泡性口炎病毒（VSV）感染。双链RNA（dsRNA）识别受体在小鼠骨髓来源的肥大细胞（BMMC）的表达分析表明，BMMC表达黑色素瘤分化相关基因5（MDA 5），蛋白激酶RNA激活，视黄酸诱导基因-I（RIG-I）和Toll样受体3。发现这些受体的表达水平在用VSV以及合成的dsRNA：聚肌苷酸-聚胞苷酸刺激肥大细胞后增加。此外，小干扰RNA分析，以确定负责肥大细胞激活VSV的受体显示，RIG-I和MDA 5参与细胞因子的产生，但不参与肥大细胞的脱粒。我们的研究结果表明，肥大细胞产生的细胞因子和趋化因子在早期感染阶段识别病毒后，通过RIG-I和MDA 5，并可能有助于抗病毒反应。这些数据提供了额外的新信息，提高了我们对涉及肥大细胞的抗病毒先天反应的理解。版权所有（C）2012 S. Karger AG，巴塞尔
To investigate the precise mechanisms of virus recognition by mast cells, the expression and functional characteristics of virus recognition receptors that lead to mast cell activation were investigated. Our results suggest that mast cells are partly responsible for the early in vivo production of antiviral cytokines and chemokines upon vesicular stomatitis virus (VSV) infection. Analysis of the expression of double-stranded RNA (dsRNA) recognition receptors in murine bone marrow-derived mast cells (BMMCs) revealed that BMMCs express melanoma differentiation-associated gene 5 (MDA5), protein kinase RNA-activated, retinoic acid-inducible gene-I (RIG-I) and Toll-like receptor 3. The expression levels of these receptors were found to increase upon stimulation of mast cells with VSV as well as synthetic dsRNA: polyinosinic-polycytidylic acid. Moreover, small interfering RNA analysis to identify the receptors responsible for mast cell activation by VSV revealed that both RIG-I and MDA5 were involved in cytokine production but not in the degranulation of mast cells. Our findings suggest that mast cells produce cytokines and chemokines in the early infection stage after recognizing viruses via RIG-I and MDA5, and may contribute to antiviral responses. These data provide additional novel information that improves our understanding of antiviral innate responses that involve mast cells. Copyright (C) 2012 S. Karger AG, Basel