Toxicological consequences of TiO2, SiC nanoparticles and multi-walled carbon nanotubes exposure in several mammalian cell types: an in vitro study
Toxicological consequences of TiO2, SiC nanoparticles and multi-walled carbon nanotubes exposure in several mammalian cell types: an in vitro study
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DOI：
10.1007/s11051-009-9694-y
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发表时间：
2010-01-01
影响因子：
2.5
通讯作者：
Carriere, Marie
中科院分区：
文献类型：
--
作者：
Barillet, Sabrina;Simon-Deckers, Angelique;Carriere, Marie
纳米技术的发展可能导致潜在有毒纳米颗粒在环境中的传播。因此，这些纳米尺寸的颗粒的毒理学引起了全世界公众和政府的关注。我们的研究集中在真核细胞对纳米颗粒暴露的体外反应。为此，我们使用了主要靶器官（肺：A549肺泡上皮细胞）或次要靶器官（肝：WIF-B 9、Can-10和肾：NRK-52 E、LLC-PK 1近端细胞）的细胞模型，即，如果纳米颗粒通过上皮屏障移位，则暴露器官。将这些细胞暴露于TiO 2、SiC纳米颗粒或多壁碳纳米管（MWCNT）。规定了纳米颗粒理化特性对各种毒理学终点（细胞毒性、活性氧生成、遗传毒性）的影响。我们的数据表明，纳米颗粒的毒性取决于它们的大小，形态和化学成分，最好的，球形的，球形的TiO 2纳米颗粒对NRK-52 E细胞更具细胞毒性，而SiC纳米颗粒几乎没有细胞毒性。多壁碳纳米管的细胞毒性既不依赖于它们的长度，也不依赖于金属杂质的存在。纳米颗粒的细胞毒性也依赖于暴露的细胞系。所有测试的纳米颗粒被细胞摄取并引起细胞内活性氧的产生。相对于遗传毒性效应，在NRK-52 E细胞中通过碱性彗星试验检测到细胞暴露于TiO 2纳米颗粒后的DNA链断裂，并在较小程度上暴露于MWCNT后，但未检测到双链断裂。这项研究的原创性在于在各种细胞系上测试的纳米材料小组。所有这些数据都可能有助于更好地了解纳米材料的毒性和对健康的危害。
The development of nanotechnologies may lead to dissemination of potentially toxic nanoparticles in the environment. Toxicology of these nano-sized particles is thus attracting attention of public and governments worldwide. Our research is focused on the in vitro response of eukaryotic cells to nanoparticles exposure. For this purpose, we used cellular models of primary target organs (lung: A549 alveolar epithelial cells), or secondary target organs (liver: WIF-B9, Can-10 and kidneys: NRK-52E, LLC-PK1 proximal cells), i.e., organs exposed if nanoparticles are translocated through epithelial barriers. These cells were exposed to TiO2, SiC nanoparticles or multi-walled carbon nanotubes (MWCNT). The influence of nanoparticles physicochemical characteristics on various toxicological endpoints (cytotoxicity, reactive oxygen species generation, genotoxicity) was specified. Our data demonstrate that nanoparticles toxicity depend on their size, morphology, and chemical composition, the finest, spherical shaped, and anatase TiO2 nanoparticles being the more cytotoxic to NRK-52E cells, while SiC nanoparticles exert almost no cytotoxicity. MWCNT cytotoxicity neither depended on their length, nor on the presence of metal impurities. Nanoparticles cytotoxicity also depended on the exposed cell line. All the tested nanoparticles were uptaken by cells and caused intracellular reactive oxygen species generation. Relative to genotoxic effects, DNA strand breaks were detected in NRK-52E cells via the alkaline comet assay after exposure of cells to TiO2 nanoparticles and to a lesser extent after exposure to MWCNT, but no double strand breaks were detected. The originality of this study lies on the panel of nanomaterials which were tested on a variety of cell lines. All these data may lead to a better understanding of nanomaterial toxicity and hazards for health.