The traA gene of the Enterococcus faecalis conjugative plasmid pPD1 encodes a negative regulator for the pheromone response.
The traA gene of the Enterococcus faecalis conjugative plasmid pPD1 encodes a negative regulator for the pheromone response.
复制标题
粪肠球菌接合质粒 pPD1 的 traA 基因编码信息素反应的负调节因子。
作者：
K. Tanimoto;H. Tomita;Y. Ike
粪肠球菌接合质粒pPD 1（59 kb）响应于信息素产生聚集物质。traA基因（962 bp）是参与pPD 1信息素反应调节的基因簇的成员。嵌合E.构建了含有来自traA基因中间部分的762-bp DNA片段的粪肠球菌自杀质粒。通过同源重组整合该嵌合质粒来破坏pPD 1 traA。急诊faecalis菌株含有pPD 1与破坏的traA表现出干燥的菌落形态，组成性结块，并在短（10分钟）交配期内以高频率转移的能力，表明pPD 1的traA产品是一个负调节器的信息素反应。
The Enterococcus faecalis conjugative plasmid pPD1 (59 kb) produces an aggregation substance in response to pheromone. The traA gene (962 bp) is a member of a gene cluster involved in the regulation of the pPD1 pheromone response. A chimeric E. faecalis suicide plasmid containing a 762-bp DNA fragment from the middle portion of the traA gene was constructed. The pPD1 traA was disrupted by integration of this chimeric plasmid via homologous recombination. The E. faecalis strain containing pPD1 with the disrupted traA exhibited dry colony morphology, constitutive clumping, and an ability to transfer at high frequencies in a short (10-min) mating period, indicating that the traA product of pPD1 is a negative regulator for the pheromone response.