Multianalyte microphysiometry as a tool in metabolomics and systems biology
Multianalyte microphysiometry as a tool in metabolomics and systems biology
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DOI：
10.1016/j.jelechem.2005.11.024
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发表时间：
2006-02-15
影响因子：
4.5
通讯作者：
Cliffel, DE
中科院分区：
文献类型：
--
作者：
Eklund, SE;Snider, RM;Cliffel, DE
测定细胞代谢分析物的时间通量有望彻底改变代谢组学和系统生物学的研究。多分析物微生理计（MMP）是一种能够测量细胞外氧、葡萄糖、乳酸和酸的流量变化的仪器，是提取细胞代谢信息的有用工具。应用程序进行审查，在三个领域：生物防御剂的歧视，通过监测响应蓖麻毒素，肉毒杆菌和霍乱毒素，以及炭疽保护性抗原（PA）的代谢通量的变化;癌细胞研究有关缺氧和细胞增殖，以及短期的环境因素对细胞种群的影响。未来的发展方向包括引入额外的电极，改进时间和空间代谢通量分析，以及细胞室和传感器技术的小型化，以实现药物筛选和发现中更具成本效益和高通量的应用。(c)2005 Elsevier B. V.保留所有权利。
The determination of temporal fluxes of cellular metabolic analytes promises to revolutionize the study of metabolomics and systems biology. A review Of Our work oil the multianalyte microphysiometer (MMP), an instrument capable of measuring extracellular changes in the flux rates of oxygen, glucose, lactate, and acid, is presented as a useful tool in extracting Cellular metabolic information. Applications are reviewed in three areas: biodefense agent discrimination by monitoring changes in metabolic fluxes in response to ricin, botulinum and cholera toxins, as well as anthrax protective antigen (PA); cancer cell studies in relation to hypoxia and cell proliferation, and the short term effects of environmental agents on cell Populations. Future directions include the incorporation of additional electrodes, improvements in temporal and spatial metabolic flux analysis, and miniaturization of the cell chamber and sensor technology for more cost effective and high-through put applications in drug screening and discovery. (c) 2005 Elsevier B.V. All rights reserved.