The causal deletions in the second exon of An-3 closely associated with awn development and rice yield
The causal deletions in the second exon of An-3 closely associated with awn development and rice yield
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An-3第二外显子的因果缺失与芒发育和水稻产量密切相关
DOI：
10.1007/s13258-017-0587-7
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发表时间：
2017-11-01
期刊：
影响因子：
2.1
通讯作者：
Qin, Baoxiang
中科院分区：
文献类型：
--
作者：
Liu, Lilong;Li, Yunzhen;Qin, Baoxiang
芒是水稻驯化过程中的重要性状之一。为了了解稻芒的发育及其在水稻驯化中的作用，我们利用广西长芒野生稻（GXCWR，Oryza rufipogon Griff.）基因组片段渗入的染色体片段替换系CSSL138，将芒发育的主效QTL An-3初步定位到短芒籼稻品种93-11的遗传背景中。然后将 An-3 精细定位到 8 号染色体的 7 kb 区域。表皮图案因子样蛋白基因被鉴定为与该 QTL 相对应的单个候选基因。 An-3被证明是RAE2和GAD1的等位基因，对千粒重、粒长和长宽比具有负调控作用。与 CSSL138 的 An-3 编码序列相比，在 93-11 和日本晴的第二外显子中分别发现了 2 bp 和 4 bp 移码缺失。综上所述，我们的研究结果提供了普通野生稻和栽培稻之间An-3等位基因的有价值的自然变异，这将有助于阐明芒发育机制并促进an-3在水稻产量性状遗传改良中的应用。
Awn is one of important traits during rice domestication. To understand the development of rice awn and the roles it played in rice domestication, we preliminary mapped a major QTL An-3 for awn development using chromosome segment substitution line CSSL138 developed by introgressed genomic fragments of long-awned Guangxi common wild rice (GXCWR, Oryza rufipogon Griff.) into genetic background of short-awned indica cultivar 93–11. An-3 was then fine mapped to a 7-kb region of chromosome 8. An epidermal patterning factor-like protein gene was identified as the single candidate gene corresponding to this QTL. An-3 was showed to be an allele of RAE2 and GAD1, and negatively regulated 1000-grains weight, grain length, and length–width ratio. Comparing with the coding sequences of An-3 from CSSL138, a 2- and 4-bp frame-shift deletions in the second exon were identified in 93–11 and Nipponbare, respectively. Taken together, our results provide valuable natural variation in the alleles of An-3 between common wild rice and cultivated rice, which will be helpful in clarifying the mechanism of awn development and promoting the application of an-3 in genetic improvement of rice yield traits.