Structural changes and fate of crystalloplastids during growth of calcium oxalate crystal idioblasts in Japanese yam (Dioscorea japonica thunb.) tubers
Structural changes and fate of crystalloplastids during growth of calcium oxalate crystal idioblasts in Japanese yam (Dioscorea japonica thunb.) tubers
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DOI：
10.1626/pps.7.283
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发表时间：
2004-09-01
影响因子：
2.5
通讯作者：
Miyake, H
中科院分区：
文献类型：
--
作者：
Kawasaki, M;Taniguchi, M;Miyake, H
用透射电镜观察了山药块茎草酸钙晶体异细胞生长过程中质体结构的变化。在块茎发育过程中，块茎皮层中发育出的异细胞体积大、椭圆形，含有大量的质体。结晶质体具有新颖的形态特征。在晶体形成过程中，在晶体质体中形成单个或多个明显的电子半透明部分，无膜结构。巧合的是，电子致密部分包含质体核糖体和管状膜形成在晶质体的外围。在晶体形成过程中，电子不透明部分逐渐增大，电子致密部分逐渐消失，形成类似于小液泡或囊泡的晶体体。在晶体形成过程中，大多数这样的晶体体进入并被并入异细胞的中央液泡中。成熟异胚细胞胞质中残留的质体为类原质体的细胞器。因此，在日本山药块茎中显示了晶体体与异细胞生长的命运。结果表明，草酸钙晶体形成过程中的物质运输过程之一是质体进入异细胞中央液泡。
The structural changes of crystalloplastids during calcium oxalate crystal idioblasts; growth in Japanese yam tubers were examined by transmission electron microscopy. Idioblasts developed in the cortex of tubers during the tubers development were large and elliptic and contained many crystalloplastids. The crystalloplastids were shown to have novel morphological characterizations. The single or multiple obvious electron-translucent parts without membrane structures were formed in the crystalloplastids during the crystal formation. Coincidentally, the electron-dense parts containing plastid ribosomes and tubular membranes were formed at the periphery of crystalloplastids. During further progress of crystal formation, obvious electron-translucent parts enlarged and finally electron-dense parts disappeared, forming the crystalloplastids similar to small vacuoles and/or vesicles. The majority of such crystalloplastids entered and was incorporated into the central vacuoles of idioblasts during crystal formation. The plastids remaining in the cytoplasm of mature idioblasts were proplastid-like organelle. Thus, the fate of crystalloplastids with growth of idioblasts was shown in Japanese yam tubers. It was suggested that the incorporation of crystalloplastids into central vacuoles of the idioblasts was one of the processes of material transportation involved in calcium oxalate crystal formation.