Cytoskeleton of rat aortic smooth muscle cells. Normal conditions and experimental intimal thickening.
Cytoskeleton of rat aortic smooth muscle cells. Normal conditions and experimental intimal thickening.
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大鼠主动脉平滑肌细胞的细胞骨架。
DOI：
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发表时间：
1984
期刊：
影响因子：
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通讯作者：
G. Gabbiani
中科院分区：
文献类型：
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作者：
O. Kocher;O. Skalli;W. Bloom;G. Gabbiani
用电子显微镜、间接免疫荧光、十二烷基硫酸钠-聚丙烯酰胺凝胶密度分析、等电聚焦和双向凝胶等方法，研究了正常情况下和血管内皮损伤后15天或75天大鼠主动脉中膜和内膜中肌动蛋白、波形蛋白和结蛋白的分布。在正常的主动脉中膜中，几乎所有的平滑肌细胞都含有波形蛋白，其中约50%的细胞还含有结蛋白；通过双向凝胶分析肌动蛋白的亚型，发现平滑肌细胞以α-肌动蛋白为主，也有少量的β-肌动蛋白，很少有γ-肌动蛋白。内皮损伤后15天，迁移到内膜的细胞与正常的中膜平滑肌细胞相比，肌动蛋白和结蛋白的含量减少，而波形蛋白的含量增加。此外，β-肌动蛋白成为主要的肌动蛋白亚型，并出现大量的伽马-肌动蛋白，而α-肌动蛋白减少。内皮损伤后75天，再生的内皮细胞已修复损伤。损伤后15天内膜平滑肌细胞数量较少，其细胞骨架成分的组织结构已基本恢复到正常状态。在内皮损伤后15天和75天，内膜增厚下的主动脉中层细胞骨架元素均未见明显改变。
The organization of actin, vimentin, and desmin in smooth muscle cells of rat aortic media and intima under normal conditions and 15 or 75 days after endothelial injury has been studied by means of electron microscopy, indirect immunofluorescence, densitometric analysis of sodium dodecyl sulfate-polyacrylamide gels, isoelectric focusing, and bidimensional gels. In the normal aortic media, practically all smooth muscle cells contain vimentin, and about 50% of them contain, in addition, desmin; upon analysis of actin isotypes by bidimensional gels, smooth muscle cells show a predominance of alpha-actin, some beta-actin, and very little gamma-actin. Fifteen days after endothelial injury, cells that have migrated into the intima contain decreased amounts of actin and desmin and increased amounts of vimentin compared with normal medial smooth muscle cells. Moreover, beta-actin becomes the predominant actin isotype and significant amounts of gamma-actin appear, whereas alpha-actin decreases. Seventy-five days after endothelial injury, regenerated endothelial cells have repaired the injury. Intimal smooth muscle cells are less numerous than 15 days after injury, and the organization of their cytoskeletal elements has reverted almost to normal conditions. At both 15 and 75 days after endothelial injury, no significant changes of cytoskeletal elements are seen in the aortic media underlying intimal thickenings.