Effects of phenethanolamines and propranolol on the proliferation of cultured chick breast muscle satellite cells.
Effects of phenethanolamines and propranolol on the proliferation of cultured chick breast muscle satellite cells.
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苯乙醇胺和普萘洛尔对培养鸡胸肌卫星细胞增殖的影响。
DOI：
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发表时间：
1990
影响因子：
3.3
通讯作者：
W. Bergen
中科院分区：
文献类型：
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作者：
A. L. Grant;W. Helferich;R. Merkel;W. Bergen
通过 Percoll 密度梯度分级技术从 20 天胚胎鸡胸肌中分离卫星细胞。这些细胞的培养产生至少 89% 的融合（肌管细胞核数/总细胞核数）。当培养基中含有成纤维细胞生长因子 (FGF)（25 至 200 ng/ml）时，培养的卫星细胞的增殖（以肌管细胞核数量表示）以剂量依赖性方式增加。使用类似的培养物来检查莱克多巴胺和异丙肾上腺素对卫星细胞增殖的影响。初次接种后24小时，将莱克多巴胺和异丙肾上腺素添加至培养基(10(-11)至10(-4)M)。 72小时处理期后，去除处理并更换为培养基以促进融合48小时。然后将细胞固定并染色，并对肌管和总细胞核进行计数。在后来的实验中，与对照培养物中观察到的相比，莱克多巴胺和异丙肾上腺素分别使肌管细胞核数量增加（P 小于 0.01）2.3 倍和 2.1 倍。总核数也观察到类似的差异。当普萘洛尔（一种 β 肾上腺素能拮抗剂）与相应激动剂以 10(-5) M 一起加入时，用 10(-6) M 莱克多巴胺或异丙肾上腺素处理的培养物中观察到的肌管细胞核数量分别减少了 25.4 和 23.6%（P 小于 0.01）。这些结果表明，莱克多巴胺和异丙肾上腺素各自增强培养物中鸡卫星细胞的增殖活性，并且β肾上腺素能受体介导这种增殖作用。
Satellite cells were isolated from 20-d embryonic chick breast muscle via a Percoll density gradient fractionation technique. Culturing of these cells gave rise to at least 89% fusion (myotube nuclei number/total nuclei number). Proliferation of cultured satellite cells (indicated by myotube nuclei number) was increased in a dose-dependent manner when fibroblast growth factor (FGF) was included in the medium (25 to 200 ng/ml). Similar cultures were used to examine the effects of ractopamine and isoproterenol on satellite cell proliferation. Ractopamine and isoproterenol were added to culture medium (10(-11) to 10(-4) M) 24 h after initial plating. After a 72-h treatment period, the treatments were removed and replaced with a medium to promote fusion for 48 h. Cells then were fixed and stained, and myotube and total nuclei were counted. In later experiments, ractopamine and isoproterenol each increased (P less than .01) myotube nuclei number vs that observed in control cultures by 2.3 and 2.1 times, respectively. Similar differences were observed with total nuclei number. The number of myotube nuclei observed in cultures treated with 10(-6) M ractopamine or isoproterenol was reduced (P less than .01) by 25.4 and 23.6%, respectively, when propranolol, a beta adrenergic antagonist, was included at 10(-5) M with the respective agonist. These results indicate that ractopamine and isoproterenol each enhance the proliferative activity of chick satellite cells in culture and that the beta adrenergic receptor mediates this proliferative effect.