Rhizoctonia solani infection in two rice lines increases mRNA expression of metabolic enzyme genes in glycolytic, oxidative pentose phosphate pathways and secondary metabolism
Rhizoctonia solani infection in two rice lines increases mRNA expression of metabolic enzyme genes in glycolytic, oxidative pentose phosphate pathways and secondary metabolism
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两个水稻品系中立枯丝核菌感染增加了糖酵解、氧化戊糖磷酸途径和次生代谢中代谢酶基因的 mRNA 表达
DOI：
10.11248/jsta.54.119
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
A. Nose
中科院分区：
文献类型：
--
作者：
M. Mutuku;A. Nose
使用两个水稻品系，即抗立枯丝核菌水稻品系2F 18 -7-32 (32)和易感水稻品系2F 21 -21-29 (29)，检查了立枯丝核菌感染样品中30个代谢酶基因的mRNA表达的时间过程的变化。它们由糖酵解途径的八个代谢酶基因组成；氧化戊糖磷酸途径（OPPP）的三个代谢酶基因和还原性戊糖磷酸循环（RPPC）的六个代谢酶基因；蔗糖磷酸酶（SPase，EC 3.1.3.24），参与蔗糖合成；参与淀粉合成的ADP-葡萄糖焦磷酸化酶（ADPase，EC 2.7.7.9）和淀粉合酶（SS，EC 2.4.1.21）；三羧酸（TCA）循环的六个代谢酶基因和次级代谢的四个代谢酶基因。结果表明，mRNA 表达的时间过程在 1 dpi 发生显着变化，并且糖酵解酶的 mRNA 表达发生显着变化。 6-磷酸果糖激酶、磷酸甘油酸激酶、烯醇化酶和丙酮酸激酶以及次级代谢酶；与感病水稻品系相比，抗性水稻品系感染立枯丝核菌的样品中 3-脱氧-D-阿拉伯-庚酮酸-7-磷酸合酶和苯丙氨酸解氨酶显着升高（P < 0.05）。结果表明，立枯丝核菌感染导致糖酵解途径、OPPP、TCA 循环和次级代谢的激活。 RPPC 基因、ADPase 和 SS 的 mRNA 表达的时间进程表明，在抗性和易感水稻品系中，感染 solani 的样品中淀粉合成较低。表明立枯丝核菌感染与糖酵解途径、OPPP、次生代谢和TCA循环的激活以及低淀粉合成有关。
Using two rice lines, namely 2F 18 -7-32 (32) a Rhizoctonia solani - resistant rice line and one, 2F 21 -21-29 (29) a susceptible rice line, changes in the time course of mRNA expression of 30 metabolic enzyme genes in R. solani- infected samples were examined. These consisted of eight metabolic enzyme genes of the glycolytic pathway; three metabolic enzyme genes of the oxidative pentose phosphate pathway (OPPP), and six metabolic enzyme genes of the reductive pentose phosphate cycle (RPPC); sucrose phosphatase (SPase, EC 3.1.3.24), participating in sucrose synthesis; ADP-glucose pyrophosphorylase (ADPase, EC 2.7.7.9) and starch synthase (SS, EC 2.4.1.21) which are involved in starch synthesis; six metabolic enzyme genes of the tricarboxylic acid (TCA) cycle and four metabolic enzyme genes of the secondary metabolism. The results showed that significant changes in the time course of mRNA expression occurred at 1 dpi and that mRNA expression of glycolytic enzymes; 6-phosphofructokinase, phosphoglycerate kinase, enolase and pyruvate kinase and secondary metabolism enzymes; 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase and phenylalanine ammonialyase was significantly higher ( P < 0.05) in the R. solani- infected samples of the resistant rice line, compared to that of the susceptible rice line. The results suggested that R. solani infection led to activation of the glycolytic pathway, OPPP, TCA cycle and secondary metabolism. Time course of mRNA expression of RPPC genes, ADPase and SS suggested that starch synthesis was low in R. solani- infected samples of both R. solani- resistant and susceptible rice lines. It appeared that R. solani infection was associated with the activation of the glycolytic pathway, OPPP, secondary metabolism and TCA cycle and low starch synthesis.