ZOLA-3D allows flexible 3D localization microscopy over an adjustable axial range
ZOLA-3D allows flexible 3D localization microscopy over an adjustable axial range
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DOI：
10.1038/s41467-018-04709-4
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发表时间：
2018-06-19
影响因子：
16.6
通讯作者：
Zimmer, Christophe
中科院分区：
文献类型：
--
作者：
Aristov, Andrey;Lelandais, Benoit;Zimmer, Christophe
单分子定位显微镜可以利用正常或工程点扩散函数（PSF）的轴向变化，无需扫描即可生成3D超分辨率图像。然而，这些方法在扩展轴向范围内的成功实施仍然具有挑战性。我们提出了3D Zernike优化定位方法（ZOLA-3D），这是一种易于使用的计算和光学解决方案，可在可调轴向范围内实现最佳分辨率。我们使用ZOLA-3D来展示整个细胞核或细胞中线粒体、核孔和微管的3D超分辨率成像，深度可达5 μ m。
Single molecule localization microscopy can generate 3D super-resolution images without scanning by leveraging the axial variations of normal or engineered point spread functions (PSF). Successful implementation of these approaches for extended axial ranges remains, however, challenging. We present Zernike Optimized Localization Approach in 3D (ZOLA-3D), an easy-to-use computational and optical solution that achieves optimal resolution over a tunable axial range. We use ZOLA-3D to demonstrate 3D super-resolution imaging of mitochondria, nuclear pores and microtubules in entire nuclei or cells up to similar to 5 mu m deep.