The Genome of Armadillidium vulgare (Crustacea, Isopoda) Provides Insights into Sex Chromosome Evolution in the Context of Cytoplasmic Sex Determination
The Genome of Armadillidium vulgare (Crustacea, Isopoda) Provides Insights into Sex Chromosome Evolution in the Context of Cytoplasmic Sex Determination
复制标题
DOI：
10.1093/molbev/msz010
复制
发表时间：
2019-04-01
影响因子：
10.7
通讯作者：
Cordaux, Richard
中科院分区：
文献类型：
--
作者：
Chebbi, Mohamed Amine;Becking, Thomas;Cordaux, Richard
陆生等足类犰狳是研究动物性别决定和共生进化的原始模型。其性别可以由 ZW 性染色体或女性化沃尔巴克氏体细菌内共生体决定。在这里，我们报告了 A. vulgare ZW 雌性基因组的序列和分析。 1.72 GB 组装体的一个显着特征是重复序列丰富（占基因组的 68%）。我们表明，Z 和 W 性染色体在分子水平上本质上是未分化的，并且 W 特异性区域非常小（最多数百千碱基）。我们的结果表明，重组抑制即使有的话，也没有传播到距性别决定基因座很远的地方。这与 A. vulgare 拥有进化上年轻的性染色体是一致的。我们对 A. vulgare 基因组中的多个 Wolbachia 核插入片段进行了表征，其中没有一个与 W 特异性区域相关。我们还鉴定了一些可能参与性别决定或性分化途径的候选基因。 A. vulgare 基因组是研究甲壳类动物生物学和进化的资源，甲壳类动物是最特殊和最具代表性的后生动物类群之一。
The terrestrial isopod Armadillidium vulgare is an original model to study the evolution of sex determination and symbiosis in animals. Its sex can be determined by ZW sex chromosomes, or by feminizing Wolbachia bacterial endosymbionts. Here, we report the sequence and analysis of the ZW female genome of A. vulgare. A distinguishing feature of the 1.72 gigabase assembly is the abundance of repeats (68% of the genome). We show that the Z and W sex chromosomes are essentially undifferentiated at the molecular level and the W-specific region is extremely small (at most several hundreds of kilobases). Our results suggest that recombination suppression has not spread very far from the sex-determining locus, if at all. This is consistent with A. vulgare possessing evolutionarily young sex chromosomes. We characterized multiple Wolbachia nuclear inserts in the A. vulgare genome, none of which is associated with the W-specific region. We also identified several candidate genes that may be involved in the sex determination or sexual differentiation pathways. The A. vulgare genome serves as a resource for studying the biology and evolution of crustaceans, one of the most speciose and emblematic metazoan groups.