Optimum detection times for bacteria and yeast species with the BACTEC 9120 aerobic blood culture system:: Evaluation for a 5-year period in a Turkish university hospital
Optimum detection times for bacteria and yeast species with the BACTEC 9120 aerobic blood culture system:: Evaluation for a 5-year period in a Turkish university hospital
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DOI：
10.1128/jcm.41.2.819-821.2003
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发表时间：
2003-02-01
影响因子：
9.4
通讯作者：
Akgün, Y
中科院分区：
文献类型：
--
作者：
Durmaz, G;Us, T;Akgün, Y
在5年的研究期间，我们使用BACTEC 9120 （Becton Dickinson诊断仪器系统）血液培养系统跟踪并记录了血培养阳性的时间。选择了7天的潜伏期方案，共评估了11,156个血培养。在3676份标本中分离出具有临床意义的微生物（32.95%）。革兰氏阳性和阴性细菌分离率分别为41.07和44.88%。14.03%的病原菌为酵母菌。假阳性率和阴性率都很低（分别为0.1和0.3%）。所有病原菌的平均检测时间为19.45 h。酵母、非发酵革兰氏阴性菌和梅利氏布鲁氏菌均在5 d内分离得到。考虑到这些数据，我们决定在我们的实验室建立一个5天的潜伏期方案，而不是通常使用的7天。
We tracked and documented the time of positivity of blood cultures by using the BACTEC 9120 (Becton Dickinson Diagnostic Instrument Systems) blood culture system over a 5-year study period. A 7-day protocol of the incubation period was selected, and a total of 11,156 blood cultures were evaluated. The clinically significant microorganisms (32.95%) were isolated in 3,676 specimens. Gram-positive and -negative bacterial isolation rates were found to be 41.07 and 44.88%, respectively. Yeasts were found in 14.03% of all pathogens. Both the false-positivity and -negativity rates were very low (0.1 and 0.3%, respectively). The mean detection times for all of the pathogens were determined to be 19.45 h. Yeasts, nonfermentative gram-negative bacteria, and Brucella melitensis strains were isolated within 5 days. By taking these data into account, we decided to establish a 5-day-incubation protocol in our laboratory instead of the 7 days that are commonly used.