Response of Chinese hamster V79 multicellular spheroids exposed to high-energy carbon ions
Response of Chinese hamster V79 multicellular spheroids exposed to high-energy carbon ions
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DOI：
10.1667/rr3113
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发表时间：
2004-02-01
影响因子：
3.4
通讯作者：
Mueller-Klieser, W
中科院分区：
文献类型：
--
作者：
Staab, A;Zukowski, D;Mueller-Klieser, W
在不同氧气氛中，在搅拌介质中用（1）227 MeV/核子C-12（+6）离子辐照直径为200 +/- 30 μ m（+/- SD）的中国仓鼠V79- 379 A球状体（平台区域）以在治疗期间对进入通道中的组织进行建模，（2）扩展布拉格峰中的碳离子对目标体积中的组织进行建模，或（3）X射线作为参考模态。模式（1）和（3）的细胞存活曲线相似，表明不存在接触效应，存在明显的氧效应，氧增强比（OER）分别为2.8和2.9。相比之下，模式（2）中的氧效应显著较小，OER为1.4。在正常或限制供氧条件下（外部pO（2）= 145或0 mmHg），布拉格峰照射的相对生物学效应（RBE）分别为2.1或4.3。即使在pO（2）= 0 mmHg时，这种模式也能诱导细胞凋亡和G（2）/M期细胞的剂量依赖性积累。处理与未处理的球状体的体积比表现出循环变化后，重粒子处理，不能直接归因于细胞周期同步。总之，结果表明，扩展布拉格峰中的碳离子比常规X射线更有效，特别是在缺氧条件下。(C)2004年，辐射研究学会
Chinese hamster V79-379A spheroids 200 +/- 30 mum (+/- SD) in diameter were irradiated in agitated medium in different oxygen atmospheres with (1) 227 MeV/nucleon C-12(+6) ions (plateau region) to model tissue in the entrance channel during therapy, (2) carbon ions in the extended Bragg peak modeling tissue in the target volume, or (3) X rays as a reference modality. Cell survival curves were similar for modes (1) and (3), indicating the absence of a contact effect and the presence of a pronounced oxygen effect with oxygen enhancement ratios (OERs) of 2.8 and 2.9, respectively. In contrast, the oxygen effect was substantially smaller in mode (2) with an OER of 1.4. Under normal or restricted oxygen supply conditions (external pO(2) = 145 or 0 mmHg), the relative biological effectiveness (RBE) was 2.1 or 4.3, respectively, for Bragg-peak irradiation. This modality induced apoptosis and a dose-dependent accumulation of cells in G(2)/M phase even at pO(2) = 0 mmHg. The volume ratios of treated to untreated spheroids exhibited cyclic variations after heavy-particle treatment that were not directly attributable to cell cycle synchronization. In summary, the results suggest that carbon ions in the extended Bragg peak are more effective than conventional X rays, particularly under hypoxic conditions. (C) 2004 by Radiation Research Society