Repair of dural defects with electrospun bacterial cellulose membranes in a rabbit experimental model
Repair of dural defects with electrospun bacterial cellulose membranes in a rabbit experimental model
复制标题
电纺细菌纤维素膜修复兔实验模型中的硬脑膜缺损
DOI：
10.1016/j.msec.2020.111246
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发表时间：
2020-12-01
影响因子：
7.9
通讯作者：
Chen, Shi-wen
中科院分区：
文献类型：
--
作者：
Jing, Yao;Ma, Xia;Chen, Shi-wen
为探讨静电纺细菌纤维素（EBC）膜修复兔硬脑膜缺损的优点及机制，进行了一系列体内外实验。与普通细菌纤维素（BC）膜相比，EBC膜的纤维结构更加分散、规整，孔隙率和持水性更好，降解性能也更上级。然而，生物力学性能略有下降。结果表明，BC和EBC膜对小鼠成纤维细胞的增殖和凋亡影响不大。BC和EBC膜修复后兔模型无感染、脑脊液漏、癫痫和脑肿胀等并发症。采用实时定量聚合酶链反应（RT-qPCR）和蛋白质印迹法，EBC组的早期炎症反应低于BC组，接近自体硬脑膜组。组织学观察和western blot显示，EBC组胶原纤维均匀分布于膜外侧，脑组织粘连和硬膜外瘢痕较少。与普通BC膜相比，EBC膜具有更好的生物物理性能和生物相容性。有望成为硬脑膜损伤修复的一种合适的替代材料。
To evaluate the advantages and mechanisms involved in repairing rabbit dural defect with a novel electrospun bacterial cellulose (EBC) membrane, a series of experiments were carried out in vitro and in vivo. Compared with common bacterial cellulose (BC) membrane, a more dispersed and regular fiber structure and a better porosity and water holding capacity were found in the EBC membrane, which also had superior degradability. However, the biomechanical properties were slightly decreased. The results demonstrated that BC and EBC membranes had little effect on proliferation and apoptosis of mouse fibroblast cells. There were no complications such as infection, cerebrospinal fluid leakage, epilepsy and brain swelling after BC and EBC membrane repairs in rabbit models. Using real-time quantitative polymerase chain reaction (RT-qPCR) and western blot, the early inflammatory reactions in the EBC group were shown to be lower than in the BC group, and were close to the autologous dura mater group. Histological observations and western blot revealed more collagen fibers evenly distributed on the outer side of EBC membranes than in the BC and unpatched groups, and fewer brain tissue adhesions and epidural scars were found in the EBC group. Compared with common BC membrane, the EBC membrane had better biophysical properties and biocompatibility. It is expected to be a suitable alternative material for the repair of damaged dura mater.