Template-synthesized protein nanotubes
Template-synthesized protein nanotubes
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DOI：
10.1021/nl048305p
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发表时间：
2005-02-01
期刊：
影响因子：
10.8
通讯作者：
Martin, CR
中科院分区：
文献类型：
--
作者：
Hou, SF;Wang, JH;Martin, CR
描述了一种用于在纳米孔氧化铝模板膜的孔内制备蛋白质纳米管的逐层沉积策略。这种方法需要交替地将模板膜暴露于所需蛋白质的溶液，然后暴露于戊二醛的溶液，戊二醛作为交联剂将蛋白质层保持在一起。组成纳米管壁的蛋白质层的数量可以通过改变交替的蛋白质/戊二醛循环的数量来随意控制。在孔壁上沉积了所需数量的层之后，可以溶解氧化铝模板以释放蛋白质纳米管。我们在这里表明，以这种方式制备的葡萄糖氧化酶纳米管催化葡萄糖氧化和血红蛋白纳米管保留其血红素电活性。此外，对于葡萄糖氧化酶纳米管，酶活性随着纳米管壁厚的增加而增加。
A layer-by-layer deposition strategy for preparing protein nanotubes within the pores of a nanopore alumina template membrane is described. This method entails alternately exposing the template membrane to a solution of the desired protein and then to a solution of glutaraldehyde, which acts as cross-linking agent to hold the protein layers together. The number of layers of protein that make up the nanotube walls can be controlled at will by varying the number of alternate protein/glutaraldehyde cycles. After the desired number of layers have been deposited on the pore walls, the alumina template can be dissolved to liberate the protein nanotubes. We show here that glucose oxidase nanotubes prepared in this way catalyze glucose oxidation and that hemoglobin nanotubes retain their heme electroactivity. Furthermore, for the glucose oxidase nanotubes, the enzymatic activity increases with the nanotube wall thickness.