Does Cellular Hydrogen Peroxide Diffuse or Act Locally?
Does Cellular Hydrogen Peroxide Diffuse or Act Locally?
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DOI：
10.1089/ars.2010.3539
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发表时间：
2011-01-01
影响因子：
6.6
通讯作者：
Belousov, Vsevolod V.
中科院分区：
文献类型：
--
作者：
Mishina, Natalia M.;Tyurin-Kuzmin, Pyotr A.;Belousov, Vsevolod V.
理解氧化还原信号需要细胞内过氧化氢（H2 O2）时空分布的数据。荧光报告分子HyPer是H2 O2成像的强大工具。然而，在整个核质区室中的Hyper的快速扩散不允许在微米尺度上可视化H2 O2梯度。在这里，我们显着提高了H2 O2成像的空间分辨率，通过应用亚细胞质靶向的Hyper。膜附着的报告分子鉴定了暴露于生长因子的细胞的细胞质内升高的H2 O2水平的“微区”。我们证明，扩散的H2 O2在细胞质中是非常有限的，提供证据表明，H2 O2的行为在细胞内的本地。抗氧化剂。氧化还原信号。14，1-7。
Understanding of redox signaling requires data on the spatiotemporal distribution of hydrogen peroxide (H2O2) within the cell. The fluorescent reporter HyPer is a powerful instrument for H2O2 imaging. However, rapid diffusion of HyPer throughout the nucleocytoplasmic compartment does not allow visualization of H2O2 gradients on the micrometer scale. Here we dramatically improved the spatial resolution of H2O2 imaging by applying subcytoplasmic targeting of HyPer. The membrane-attached reporters identified "microdomains'' of elevated H2O2 levels within the cytoplasm of the cells exposed to growth factors. We demonstrate that diffusion of H2O2 across the cytoplasm was strongly limited, providing evidence that H2O2 acts locally inside cells. Antioxid. Redox Signal. 14, 1-7.