Identification of a human subcortical maternal complex
Identification of a human subcortical maternal complex
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人类皮层下母性复合体的鉴定
DOI：
10.1093/molehr/gau116
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发表时间：
2015-04-01
影响因子：
4
通讯作者：
Li, Lei
中科院分区：
文献类型：
--
作者：
Zhu, Kai;Yan, Liying;Li, Lei
母体效应基因在早期胚胎发育中起着至关重要的作用。然而，母性效应基因调控哺乳动物早期胚胎发育的机制在很大程度上仍不清楚。最近，我们发现了一个皮质下母体复合体(SCMC)，它由至少四个由Mater、Floped、Tle6和Filia编码的蛋白质组成，对小鼠着床前发育至关重要。本研究表明，人SCMC同源基因(NLRP5、OOEP、TLE6和KHDC3L)在人胚胎卵巢的卵母细胞中特异表达。这种复合体的蛋白质共同定位于人类卵母细胞和早期胚胎的皮质下。此外，当SCMC蛋白在细胞系中共表达时，它们在物理上相互作用。这些结果表明，人NLRP5、OOEP、TLE6和KHDC3L在智人卵母细胞和早期胚胎中以复合体的形式存在。考虑到SCMC在小鼠早期胚胎发生中的重要作用，人SCMC的特性将为研究人类早期胚胎发育提供基础，并将对人类女性不孕或反复自然流产具有临床意义。
Maternal effect genes play essential roles in early embryonic development. However, the mechanisms by which maternal effect genes regulate mammalian early embryonic development remain largely unknown. Recently, we identified a subcortical maternal complex (SCMC) that is composed of at least four proteins encoded by Mater, Floped, Tle6 and Filia and is critical for mouse preimplantation development. The present study demonstrates that human SCMC homologous genes (NLRP5, OOEP, TLE6 and KHDC3L) are specifically expressed in the oocytes of human fetal ovaries. The proteins of this complex co-localize in the subcortex of human oocytes and early embryos. Furthermore, the SCMC proteins physically interact with each other when they are co-expressed in cell lines. These results indicate that human NLRP5, OOEP, TLE6 and KHDC3L function as a complex in the oocytes and early embryos of Homo sapiens. Considering the important roles of the SCMC in mouse early embryogenesis, the characterization of the human SCMC will provide a basis for investigating human early embryonic development and will have clinical implications in human female infertility or recurrent spontaneous abortion.