Rapid formation of advanced glycation end products by intermediate metabolites of glycolytic pathway and polyol pathway
Rapid formation of advanced glycation end products by intermediate metabolites of glycolytic pathway and polyol pathway
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DOI：
10.1006/bbrc.1996.1695
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发表时间：
1996-11-12
影响因子：
3.1
通讯作者：
Hotta, N
中科院分区：
文献类型：
--
作者：
Hamada, Y;Araki, N;Hotta, N
为了澄清糖酵解途径和多元醇途径的中间代谢产物在生理条件下的非酶糖基化中的作用，我们孵育牛血清白蛋白与两种途径的中间体在微摩尔范围内，以及与20 mmol/l葡萄糖，并观察到晚期糖基化终产物（AGEs）的形成。我们发现磷酸丙糖、甘油醛和一种新的多元醇途径相关代谢产物3-磷酸果糖沿着其分解产物3-脱氧葡萄糖酮是非常有效的糖化剂，在接近生理浓度下与白蛋白孵育后，早在24小时就产生大量的AGE，而20 mmol/l葡萄糖孵育两周后产生痕量的AGE。结果沿着先前糖尿病状态中中间体水平增加的证据可能表明，中间代谢物而不是葡萄糖有助于糖尿病组织中糖化的增强，尽管与葡萄糖相比，中间代谢物的浓度低得多。(C)出版社：Academic Press，Inc.
To clarify roles of intermediate metabolites of the glycolytic pathway and the polyol pathway in nonenzymatic glycation under physiological conditions, we incubated bovine serum albumin with intermediates of both pathways in the micromolar range as well as with 20 mmol/l glucose, and observed the formation of advanced glycation end products (AGEs). We found that triose phosphates, glyceraldehyde, and a novel polyol pathway-related metabolite, fructose 3-phosphate along with its breakdown product, 3-deoxyglucosone were extremely potent glycating agents that at nearly physiological concentrations on incubation with albumin produced substantial amounts of AGEs as early as 24 hours, while 20 mmol/l glucose afforded trace amounts of AGEs after two week incubation. The results along with the previous evidence of the increased level of intermediates in diabetic states may suggest that the intermediate metabolites rather than glucose contribute to enhanced glycation in diabetic tissues, inspite of the much lower concentrations compared with glucose. (C) 1996 Academic Press, Inc.