Human type 2 innate lymphoid cells disrupt skin keratinocyte tight junction barrier by IL-13
Human type 2 innate lymphoid cells disrupt skin keratinocyte tight junction barrier by IL-13
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DOI：
10.1111/all.13935
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发表时间：
2019-07-01
期刊：
影响因子：
12.4
通讯作者：
Akdis, Cezmi A.
中科院分区：
文献类型：
--
作者：
Sugita, Kazunari;Altunbulakli, Can;Akdis, Cezmi A.
人2型先天淋巴样细胞通过IL-13 C破坏皮肤角质形成细胞紧密连接屏障，与ILC 2共培养的紧密连接（TJ）基因的mRNA表达。为了进一步研究ILC 2s对皮肤角质形成细胞TJ屏障的作用，我们采用定量PCR方法分析了TJ及其相关基因的mRNA表达。如图S4所示，与未刺激的NHEK相比，暴露于IL-13的NHEK显示出显着较低的TER，这表明IL-13重现了ILC 2衍生的IL-13降低的屏障完整性。[摘自文章]过敏的版权是威利-布莱克威尔的财产，其内容不得复制或通过电子邮件发送到多个网站或张贴到一个列表服务器未经版权保持器的明确书面许可。但是，用户可以打印，下载或电子邮件的文章供个人使用。这篇摘要可以删节。不保证副本的准确性。用户应参考原始出版版本的材料的完整摘要。版权适用于所有摘要。
Human type 2 innate lymphoid cells disrupt skin keratinocyte tight junction barrier by IL-13 C, mRNA expression of tight junction (TJ) genes cocultured with ILC2s. To further investigate skin keratinocyte TJ barrier by ILC2s, we analyzed the mRNA expression of TJ and related genes by quantitative PCR. As shown in Figure S4, NHEKs exposed to IL-13 showed significantly lower TER compared with unstimulated NHEKs, suggesting that IL-13 recapitulates the decreased barrier integrity by ILC2-derived IL-13.[Extracted from the article]Copyright of Allergy is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. Copyright applies to all Abstracts.