Diminished Expression Alleles for Analysis of Virulence Traits and Genetic Interactions in Candida albicans.
Diminished Expression Alleles for Analysis of Virulence Traits and Genetic Interactions in Candida albicans.
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用于分析白色念珠菌毒力特征和遗传相互作用的表达减少等位基因。
DOI：
10.1007/978-1-0716-1182-1_1
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Mitchell,AaronP
中科院分区：
文献类型：
--
作者：
Woolford,CarolA;Mitchell,AaronP
在这里，我们提出了一个协议的利益，那些谁想要看看在功能上的后果，减少表达的必需基因组成，以及能够研究的调控途径，也删除一个或两个额外的基因，该必需基因。这允许确定上位关系。
Here we present a protocol of interest to those who want to look at the functional consequences of decreasing expression of essential genes constitutively as well as being able to study the regulatory pathways of that essential gene by also deleting one or two additional genes. This allows epistasis relationships to be determined.