ANTIBODY-MEDIATED AND CELL-MEDIATED IMMUNE RESISTANCE BY GUINEA-PIGS TO ADULT AMBLYOMMA-AMERICANUM TICKS
ANTIBODY-MEDIATED AND CELL-MEDIATED IMMUNE RESISTANCE BY GUINEA-PIGS TO ADULT AMBLYOMMA-AMERICANUM TICKS
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DOI：
10.4269/ajtmh.1982.31.1285
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发表时间：
1982-01-01
影响因子：
3.3
通讯作者：
BROWN, SJ
中科院分区：
文献类型：
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作者：
BROWN, SJ
将A.阿米巴蜱。未经处理的宿主允许75-81%的蜱虫产量，518-581 mg的平均蜱虫重量和12-13天的平均蜱虫摄食期。主动致敏和挑战的宿主仅允许47%的蜱虫产量（38.9%的排斥率），充满个体的体重显著降低（66.1%重量降低），并且需要稍长的进食时间（14天）。被动致敏的宿主给予血清或从主动致敏和激发的表达抗性的豚鼠中收获的腹膜渗出液细胞（PEC）。免疫血清的接受者允许56%的蜱产量（27.3%排斥），充满个体的平均体重为376 mg（减少35.3%），平均需要15天的喂养期。免疫PEC的受体被赋予了更强的保护，仅允许50%的蜱产量（35.1%的排斥率），平均蜱重为413 mg（减少28.9%），雌性需要平均15天的喂养期才能吃饱。在雌性和雄性附着部位（62-64%），首次接触蜱虫的动物中，对蜱虫摄食的皮肤细胞反应主要由中性粒细胞引起，雌性部位的细胞反应（病变）是雄性部位的10倍。在主动致敏和挑战（抗性）宿主中，对雌性和雄性的皮肤细胞反应主要由嗜碱性粒细胞（53-59%）主导，病变大小几乎是初次喂养的20倍，表明对蜱叮咬的敏感性提高。
Naive and actively and passively sensitized guinea pigs were infused with adult A. ameircanum ticks. Naive hosts allowed for 75-81% tick yield, 518-581 mg mean tick weight and an average tick feeding period of 12-13 days. Actively sensitized and challenged hosts allowed only 47% tick yield (38.9% rejection), replete individuals were significantly reduced in weight (66.1% wt decrease) and required slightly longer time to feed (14 days). Passively sensitized hosts were given either serum or peritoneal exudate cells (PEC) harvested from actively sensitized and challenged guinea pigs which had expressed resistance. Recipients of immune serum allowed 56% tick yield (27.3% rejection), replete individuals had a mean weight of 376 mg (35.3% reduction) and required an average feeding period of 15 days. Recipients of immune PEC were conferred with stronger protection allowing only 50% tick yield (35.1% rejection) with a mean tick weight of 413 mg (28.9% reduction) and females required an average feeding period of 15 days to become replete. Cutaneous cellular responses to tick feeding in naive animals were dominated by neutrophils in both female and male attachment sites (62-64%), with the cellular response (lesion) in female sites being 10 times that of male sites. In actively sensitized and challenged (resistant) hosts, cutaneous cellular resposnes to both females and males were dominated by basophils (53-59%), and lesion size was nearly 20 times greater than in primary feedings, indicating heightened sensitivity to tick bite.