Primary Patient-Derived Cancer Cells and Their Potential for Personalized Cancer Patient Care.
Primary Patient-Derived Cancer Cells and Their Potential for Personalized Cancer Patient Care.
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DOI：
10.1016/j.celrep.2017.11.051
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发表时间：
2017-12-12
期刊：
影响因子：
8.8
通讯作者：
Benes CH
中科院分区：
文献类型：
--
作者：
Kodack DP;Farago AF;Dastur A;Held MA;Dardaei L;Friboulet L;von Flotow F;Damon LJ;Lee D;Parks M;Dicecca R;Greenberg M;Kattermann KE;Riley AK;Fintelmann FJ;Rizzo C;Piotrowska Z;Shaw AT;Gainor JF;Sequist LV;Niederst MJ;Engelman JA;Benes CH
个性化癌症治疗是基于患者的肿瘤谱系、组织病理学、表达分析和/或肿瘤DNA或RNA分析。在这里，我们的目标是开发一种患者活癌细胞的体外功能测定，可以补充这些方法。我们提出了从肿瘤活检中培养细胞的方法，并确定了与模型建立效率更高的样品类型和培养条件。为了将患者来源的细胞培养物应用于个性化护理，我们建立了一种基于免疫荧光的功能测定方法，量化混合细胞培养物中癌细胞对靶向治疗的反应。用这种方法分析患者来源的肺癌培养物，显示了对诊断使用的患者反应建模的希望。该平台应允许开展联合临床试验研究，以前瞻性地测试肿瘤活检衍生培养物的药物谱分析的价值，以指导患者护理。一种基于免疫荧光的方法来量化混合细胞培养中的药物敏感性非小细胞肺癌患者的活检培养敏感性反映临床反应Kodack等人报道了肿瘤活检和个性化医疗功能方法的癌症模型的发展。他们描述了在患者来源的混合细胞群样本中可靠地测试药物反应的能力。在这样做的过程中，他们表明患者活检培养可以预测患者的临床反应。
Personalized cancer therapy is based on a patient’s tumor lineage, histopathology, expression analyses, and/or tumor DNA or RNA analysis. Here, we aim to develop an in vitro functional assay of a patient’s living cancer cells that could complement these approaches. We present methods for developing cell cultures from tumor biopsies and identify the types of samples and culture conditions associated with higher efficiency of model establishment. Toward the application of patient-derived cell cultures for personalized care, we established an immunofluorescence-based functional assay that quantifies cancer cell responses to targeted therapy in mixed cell cultures. Assaying patient-derived lung cancer cultures with this method showed promise in modeling patient response for diagnostic use. This platform should allow for the development of co-clinical trial studies to prospectively test the value of drug profiling on tumor-biopsy-derived cultures to direct patient care. Successful culture of cancer cells from tumor biopsies An immunofluorescence-based assay to quantify drug sensitivity in mixed cell cultures NSCLC patients’ biopsy culture sensitivities reflect clinical response Kodack et al. report on the development of cancer models from tumor biopsies and technologies toward a functional approach for personalized medicine. They describe the ability to reliably test drug response in patient-derived samples of mixed cell populations. In doing so, they show that patient biopsy cultures may predict patient clinical responses.
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人角蛋白：生物学和病理学。
影响因子：
2.3
作者：
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影响因子：
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Engelman JA
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28.2
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Pauli C;Hopkins BD;Prandi D;Shaw R;Fedrizzi T;Sboner A;Sailer V;Augello M;Puca L;Rosati R;McNary TJ;Churakova Y;Cheung C;Triscott J;Pisapia D;Rao R;Mosquera JM;Robinson B;Faltas BM;Emerling BE;Gadi VK;Bernard B;Elemento O;Beltran H;Demichelis F;Kemp CJ;Grandori C;Cantley LC;Rubin MA
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Rubin MA
影响因子：
158.5
作者：
Kobayashi, S;Boggon, TJ;Halmos, B
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Halmos, B
影响因子：
64.8
作者：
Straussman, Ravid;Morikawa, Teppei;Shee, Kevin;Barzily-Rokni, Michal;Qian, Zhi Rong;Du, Jinyan;Davis, Ashli;Mongare, Margaret M.;Gould, Joshua;Frederick, Dennie T.;Cooper, Zachary A.;Chapman, Paul B.;Solit, David B.;Ribas, Antoni;Lo, Roger S.;Flaherty, Keith T.;Ogino, Shuji;Wargo, Jennifer A.;Golub, Todd R.
通讯作者：
Golub, Todd R.