Effects of acute postnatal exposure to 3,3′,4,4′-tetrachlorobiphenyl on sperm function and hormone levels in adult rats
Effects of acute postnatal exposure to 3,3′,4,4′-tetrachlorobiphenyl on sperm function and hormone levels in adult rats
复制标题
DOI：
10.1016/j.chemosphere.2003.09.006
复制
发表时间：
2004-02-01
期刊：
影响因子：
8.8
通讯作者：
Li, MH
中科院分区：
文献类型：
--
作者：
Hsu, PC;Guo, YLL;Li, MH
多氯联苯（PCBs）被认为是潜在的内分泌干扰物，因为它们能够作为雌激素、抗雌激素和致甲状腺肿剂。本研究的目的是确定3，3 '，4，4'-四氯联苯（CB 77）的急性产后处理是否会影响成年大鼠的精子功能和激素水平。雄性Sprague-Dawley大鼠在第21天通过ip注射2或20 mg/kg接受CB 77，并在第112天处死。在第112天，右侧和左侧睾丸重量显着增加，而精子计数，活力，总活动精子计数，曲线速度，平均路径速度，直线速度，和beat-cross频率的活动精子显着减少，在大鼠与20 mg/kg CB 77治疗。在用2或20 mg/kg CB 77处理的大鼠中，精子-卵母细胞穿透率显著降低。20 mg/kg CB 77处理组大鼠精子顶体反应率（ARR）明显升高。在20 mg/kg CB 77组中，促甲状腺激素水平显著增加。然而，在第112天，血清睾酮、甲状腺激素或催乳素浓度没有变化。总之，本研究表明出生后暴露于CB 77可能会影响成熟大鼠的精子发生、运动、ARR和卵母细胞的能力。这些结果表明，精子功能可能更容易或适应不太容易比甲状腺功能二恶英类PCB同系物引起的内分泌干扰。(C)2003爱思唯尔有限公司。保留所有权利。
Polychlorinated biphenyls (PCBs) are considered potential endocrine disruptors due to their ability to act as estrogens, antiestrogens and goitrogens. The aim of this study is to ascertain whether acute postnatal treatment with 3,3',4,4'-tetrachlorobiphenyl (CB 77) affects sperm function and hormone levels in adult rats. Male Sprague-Dawley rats received CB 77 by ip injection of 2 or 20 mg/kg at day 21 and sacrificed at day 112. At day 112, right and left testis weights were significantly increased, whereas sperm count, motility, total motile sperm count, curvilinear velocity, average path velocity, straight-line velocity, and beat-cross frequency for motile sperm were significantly decreased in rats treated with 20 mg/kg CB 77. Sperm-oocyte penetration rate was significantly reduced in rats treated with either 2 or 20 mg/kg CB 77. There was high sperm acrosome reaction rate (ARR) in the 20 mg/kg CB 77-treated rats. There was a significant increase in thyroid-stimulating hormone level in the 20 mg/kg CB 77 group. However, no changes were seen in serum testosterone, thyroid hormones, or prolactin concentrations at day 112. In summary, this study showed that postnatal exposure to CB 77 might affect spermatogenesis, motility, ARR, and ability of fertilizing oocytes in mature rats. These results suggest that the sperm functions may be more susceptible or adapt less readily than the thyroid functions to endocrine disruption caused by dioxin-like PCB congeners. (C) 2003 Elsevier Ltd. All rights reserved.