Molecular tools - advances, opportunities and prospects
Molecular tools - advances, opportunities and prospects
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DOI：
10.1016/j.vetpar.2005.12.002
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发表时间：
2006-03-15
影响因子：
2.6
通讯作者：
Gasser, RB
中科院分区：
文献类型：
--
作者：
Gasser, RB
现代分子技术对寄生虫学的许多基础和应用领域产生了重大影响。特别是，聚合酶链反应（PCR）偶联方法已经发现了广泛的适用性，因为它们的敏感性允许从微量的寄生虫物质中提取微量的核酸进行基因片段的酶扩增。此外，高分辨率的电泳和基因组方法也越来越实用。本文简要讨论了DNA方法在寄生虫研究中的一些进展和应用，并强调了它们在兽医研究中的实用性和潜力。介绍了在基础（系统学、种群遗传学、流行病学和生态学）和应用（诊断、预防和控制）领域具有影响的选定应用实例。重点主要是准确识别具有社会经济重要性的寄生线虫和原生动物的工具，感染诊断和使用pcr偶联突变扫描技术检测遗传变异。(c) 2005 Elsevier B.V.版权所有
Modem molecular technologies are having a substantial impact in many fundamental and applied areas of parasitology. In particular, polymerase chain reaction (PCR)-coupled approaches have found broad applicability because their sensitivity permits the enzymatic amplification of gene fragments from minute quantities of nucleic acids from tiny amounts of parasite material. Also, high-resolution electrophoretic and genomic methods are finding increased utility. This paper briefly discusses some developments and applications of DNA methods to parasites and highlights their usefulness or potential for those of veterinary importance. Selected examples of applications with implications in fundamental (systematics, population genetics, epidemiology and ecology) and applied (diagnosis, prevention and control) areas are presented. The focus is mainly on tools for the accurate identification of parasitic nematodes and protozoa of socio-economic importance, the diagnosis of infections and the detection of genetic variability using PCR-coupled mutation scanning technology. (c) 2005 Elsevier B.V. All rights reserved.