MICROINJECTIONS OF A NICOTINIC AGONIST INTO DOPAMINE TERMINAL FIELDS - EFFECTS ON LOCOMOTION
MICROINJECTIONS OF A NICOTINIC AGONIST INTO DOPAMINE TERMINAL FIELDS - EFFECTS ON LOCOMOTION
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DOI：
10.1016/0091-3057(90)90050-r
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发表时间：
1990-09-01
影响因子：
3.6
通讯作者：
WISE, RA
中科院分区：
文献类型：
--
作者：
MUSEO, E;WISE, RA
尼古丁诱导运动，这是一种与中皮质边缘多巴胺系统相关的行为。本研究确定了直接微量注射烟碱受体激动剂野靛碱到四个DA终末领域，其中烟碱受体已本地化的运动的影响：延髓核（NAS，n = 20），尾壳核（CPU，n = 9），嗅结节（OT，n = 8），内侧前额叶皮层（MPC，n = 12）。通过留置套管向雄性Long-Evans大鼠注射野靛碱（每侧每0.5 μ l 0.1、1、10和100纳摩尔）或赋形剂，并在60分钟的测试期间记录自发活动;以平衡顺序用每种剂量测试每只动物。NAS注射三个最高剂量的野靛碱增加运动相对于媒介物注射;在CPU中的注射，背对NAS，是无效的，如MPC和OT注射。这些数据支持全身尼古丁可能与NAS的多巴胺能投射相互作用以增加运动活性的观点。
Nicotine induces locomotion, a behavior associated with the mesocorticolimbic dopamine system. The present study determined the effects on locomotion of direct microinjections of the nicotinic agonist cytisine into four DA terminal fields where nicotinic receptors have been localized: nucleus accumbens (NAS, n = 20), caudate putamen (CPU, n = 9), olfactory tubercle (OT, n = 8), and medial prefrontal cortex (MPC, n = 12). Male Long-Evans rats were injected with cytisine (0.1, 1, 10 and 100 nanomoles per 0.5 .mu.l per side) or vehicle through indwelling cannulae, and locomotor activity was recorded during a 60-minute test session; each animal was tested with each dose in counterbalanced order. NAS injections of the three highest doses of cytisine increased locomotion relative to vehicle injections; injections in the CPU, dorsal to the NAS, were ineffective, as were MPC and OT injections. The data support the notion that systemic nicotine may interact with dopaminergic projections to the NAS to produce increases in locomotor activity.