Bio-Synthesized Nanoflowers and Chemically Synthesized Nanowires Zinc-Oxide induced Changes in the Redox and Protein Folding in Soybean Seedlings: a Proteomic Analysis
Bio-Synthesized Nanoflowers and Chemically Synthesized Nanowires Zinc-Oxide induced Changes in the Redox and Protein Folding in Soybean Seedlings: a Proteomic Analysis
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DOI：
10.1007/s00344-022-10728-9
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发表时间：
2022-08
影响因子：
4.8
通讯作者：
Ghazala Mustafa;Suzuna Miyagawa;M. Hasan;H. Yamaguchi;Keisuke Hitachi;K. Tsuchida;S. Komatsu
中科院分区：
文献类型：
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作者：
Ghazala Mustafa;Suzuna Miyagawa;M. Hasan;H. Yamaguchi;Keisuke Hitachi;K. Tsuchida;S. Komatsu
植物介导的纳米花合成方法开辟了氧化锌纳米花广泛应用、经济、稳定、可重复性好的新时代。为了研究生物合成纳米线（BS）和化学合成纳米线（CS NWs）氧化锌对大豆的影响，采用无凝胶/无标记蛋白质组学技术。10 ppm BS NFs和CS NWs ZnO均能提高包括下胚轴在内的根的长度和重量。BS NFs和CS NWs ZnO之间的蛋白质变化与激素代谢、蛋白质折叠和氧化还原代谢有关。热休克蛋白70 （HSP70）丰度在BS NFs ZnO中增加，而在CS NWs ZnO中没有变化。BS NFs氧化锌中抗坏血酸过氧化物酶和过氧化物还蛋白的丰度分别升高和降低。抗坏血酸和过氧化氢分别随着BS NFs和CS NWs ZnO的增加而增加。这些结果表明，BS NFs氧化锌可能通过增加HSP70积累的蛋白质折叠和过氧化氢解毒的氧化还原代谢来促进大豆生长。另一方面，CS NWs氧化锌处理大豆蛋白质折叠缺失，氧化应激增加。
Plant-mediated synthesis methods of nanoflowers (NFs) open a new epoch of extensive application, economical, exceedingly stable, and reproducible synthesis of zinc oxide (ZnO) NFs. To study the effects of bio-synthesized (BS) NFs and chemically synthesized nanowires (CS NWs) ZnO on soybean, a gel-free/ label-free proteomic technique was used. Length and weight of root including hypocotyl were enhanced by 10 ppm BS NFs and CS NWs ZnO. Oppositely changed proteins between BS NFs and CS NWs ZnO were related to hormone metabolism, protein folding, and redox metabolism. Abundance of heat shock protein 70 (HSP70) increased in BS NFs ZnO while did not change in CS NWs ZnO. Abundance of ascorbate peroxidase and peroxiredoxin increased and decreased, respectively, in BS NFs ZnO. Ascorbic acid and hydrogen peroxide increased, respectively, with BS NFs and CS NWs ZnO. These results suggest that BS NFs ZnO might improve the soybean growth by increasing the protein folding due to accumulation of HSP70 and redox metabolism through detoxification of hydrogen peroxide. On the other hand, CS NWs ZnO treated soybean experienced loss of protein folding and increased oxidative stress.