MOLECULAR-CLONING AND ANALYSIS OF CDNA SEQUENCES FOR 2 RIBOSOMAL-PROTEINS FROM ARTEMIA - THE COORDINATE EXPRESSION OF GENES FOR RIBOSOMAL-PROTEINS AND ELONGATION FACTOR-I DURING EMBRYOGENESIS OF ARTEMIA
MOLECULAR-CLONING AND ANALYSIS OF CDNA SEQUENCES FOR 2 RIBOSOMAL-PROTEINS FROM ARTEMIA - THE COORDINATE EXPRESSION OF GENES FOR RIBOSOMAL-PROTEINS AND ELONGATION FACTOR-I DURING EMBRYOGENESIS OF ARTEMIA
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DOI：
10.1111/j.1432-1033.1985.tb08968.x
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发表时间：
1985-01-01
期刊：
影响因子：
--
通讯作者：
MOLLER, W
中科院分区：
文献类型：
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作者：
MAASSEN, JA;SCHOP, EN;MOLLER, W
真核核糖体的大亚基含有与大肠杆菌的 L7/L12 相关的酸性磷蛋白。在丰年虫丰年虫中，这些蛋白质被命名为 eL 12 和 eL 12''。 c[互补] 从 cDNA 库中分离出这些蛋白质的 DNA 克隆，该 cDNA 库是通过使用来自卤虫的大小分级的富含多聚 (A) 的 RNA（8-10S 级分）和合成寡核苷酸作为引物构建的。通过杂交选择翻译和 DNA 测序来表征含有编码 eL 12 和 eL 12'' 的 DNA 序列的克隆。蛋白质 eL 12 和 eL 12'' 在其羧基末端共享 22 个氨基酸的相同肽；该蛋白质的其余部分显示出很少的序列同源性。核苷酸序列显示共同羧基末端的氨基酸使用不同的密码子，从而排除了编码两种蛋白质这部分的共同外显子。尽管 eL 12 和 eL 12'' 的主要部分的氨基酸序列存在差异，但基于疏水性和亲水性氨基酸在多肽链上的分布，这些蛋白质具有相当程度的同源性，这与两种蛋白质的相关折叠和功能一致。编码 eL 12、eL 12'' 和延伸因子 1.alpha 的 mRNA 相对水平。是在卤虫从休眠囊肿发育成无节幼体的过程中确定的。数据显示EF-1.α基因的坐标表达。和两种核糖体蛋白，排除这些相关核糖体蛋白在胚胎发生过程中基因的差异表达。 eL 12 和 eL 12'' 的基因拷贝数分析表明每种蛋白质存在一些基因。
The large subunit of eukaryotic ribosomes contains acidic phosphoproteins which are related to L7/L12 from Escherichia coli. In the brine shrimp artemia these proteins are designated eL 12 and eL 12''. c[Complementary] DNA clones were isolated for these proteins from a cDNA bank that was constructed by the use of size-fractionated poly(A)-rich RNA (8-10S fraction) from artemia and a synthetic oligonucleotide as primer. Clones containing DNA sequences coding for eL 12 and eL 12'' were characterized by hybrid-selected translation and DNA sequencing. The proteins eL 12 and eL 12'' share an identical peptide of 22 amino acids at their carboxy termini; the remaining part of the protein shows little sequence homology. The nucleotide sequences show a different codon use for the amino acids in the common carboxy terminus, thereby excluding a common exon coding for this part of both proteins. Despite the differences in amino acid sequence in the major part of eL 12 and eL 12'' the proteins have a considerable degree of homology on the basis of the distribution of hydrophobic and hydrophilic amino acids over the polypeptide chains, in agreement with a related folding and function of both proteins. Relative levels of mRNA coding for eL 12, eL 12'' and elongation factor 1.alpha. were determined during the development of artemia from a dormant cyst to a nauplius. The data show a coordinate expression of the genes for EF-1 .alpha. and both ribosomal proteins, excluding a differential expression of the genes for these related ribosomal proteins during embryogenesis. Analysis of the gene copy number for eL 12 and eL 12'' indicates the presence of a few genes for each protein.