Staphylococcal alpha toxin: a study with chronically instrumented awake sheep.
Staphylococcal alpha toxin: a study with chronically instrumented awake sheep.
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葡萄球菌α毒素：一项对长期清醒的绵羊进行的研究。
DOI：
10.1128/iai.60.9.3489-3496.1992
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发表时间：
1992
影响因子：
3.1
通讯作者：
Snapper,JR
中科院分区：
文献类型：
--
作者：
Harshman,S;Lefferts,PL;Snapper,JR
在体内反应葡萄球菌α毒素报告了15长期仪器清醒一岁羊。从总共30个实验中获得的数据被分为四类反应：在5只羊身上进行的7个实验中没有反应；加压反应，4只羊7次；流体和溶质交换，在3只羊中出现6次；还有急性心力衰竭和死亡，15只羊中有10只发生了这种情况。“无反应”表示任何测量结果变量均无变化。标记为“升压反应”的那组羊对α毒素输注的反应是肺动脉压升高，肺淋巴流动或肺力学没有变化。“肺液和溶质交换的改变”包括肺淋巴流动的增加。最后一类，导致死亡的急性左心衰的先兆是左心房压的显著升高。绵羊的阈值反应剂量约为21微克/千克。观察到一个非常陡峭的剂量-反应曲线，在没有反应的组和因急性心力衰竭而死亡的组之间只有一个狭窄的剂量窗口，15至25微克/公斤。这些研究中获得的数据表明，α毒素对绵羊的致命影响包括急性心力衰竭，这可能是由于对心肌和/或冠状血管内皮的直接毒性。
The in vivo responses to staphylococcal alpha toxin are reported for 15 chronically instrumented awake yearling sheep. The data obtained from a total of 30 experiments are grouped into four categories of response: no response, noted in seven experiments done on 5 sheep; pressor response, obtained seven times in 4 sheep; fluid and solute exchange, noted on six occasions in 3 sheep; and acute heart failure and death, which occurred in 10 of the 15 sheep. "No response" denoted no change in any of the measured outcome variables. The group of sheep labeled as showing "pressor response" responded to alpha toxin infusion with an increase in pulmonary artery pressure, unaccompanied by changes either in lung lymph flow or in lung mechanics. "Changes in lung fluid and solute exchange" involve increases in lung lymph flow. The harbinger of the last category, acute left heart failure leading to death, was a marked elevation in left atrial pressure. The threshold response dose in sheep is approximately 21 micrograms/kg. A very steep dose-response curve is observed, with only a narrow window of doses, 15 to 25 micrograms/kg, between the group showing no response and the group showing death from acute heart failure. The data obtained in these studies indicate that the lethal effects of alpha toxin in sheep include acute heart failure, which may be due to direct toxicity to heart muscle and/or the coronary vasculature endothelium.