Analysis of cytosine methylation in early generations of resynthesized Brassica napus
Analysis of cytosine methylation in early generations of resynthesized Brassica napus
复制标题
再合成甘蓝型油菜早期世代胞嘧啶甲基化分析
DOI：
10.1016/s2095-3119(15)61277-1
复制
发表时间：
2016-01-01
影响因子：
4.8
通讯作者：
Wang You-ping
中科院分区：
文献类型：
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作者：
Ran Li-ping;Fang Ting-ting;Wang You-ping
DNA甲基化是一种重要的表观遗传修饰，在许多作物的多倍化过程中起着关键作用。本研究以人工合成的甘蓝型油菜早代（F-1、S-1-S-3）、祖先亲本B. rapa和B.应用DNA甲基化敏感扩增多态性（MSAP）和高效液相色谱（HPLC）方法，对甘蓝（oleracea）多倍体化过程中的DNA甲基化状态进行了分析。在F-1中，53.4%的片段遗传自A-和C-基因组。此外，F-1代中分别有5.04%和8.87%的片段遗传自A-和C-基因组. 5.85再合成的B有0.8%的片段出现和消失。油菜。13.1%的基因位点发生了甲基化改变，即高甲基化（7.86%）和低甲基化（5.24%）。与S-1-S-3相比，在F-1中检测到最低的甲基化状态（38.7%）。在S-3中，40.32%的基因甲基化。甲基化片段的测序表明，参与多种生物过程的基因被修饰，包括转录因子，蛋白质修饰和转运蛋白。DNA甲基转移酶1和DNA甲基转移酶3在不同材料中的表达分析与DNA甲基化状态一致。这些结果通常可以促进DNA甲基化如何有助于遗传稳定性和B的改善的解剖。甘蓝型油菜在多倍体化过程中。
DNA methylation, an important epigenetic modification, serves as a key function in the polyploidization of numerous crops. In this study, early generations of resynthesized Brassica napus (F-1, S-1-S-3), ancestral parents B. rapa and B. oleracea were analyzed to characterize their DNA methylation status during polyploidization, applying DNA methylation-sensitive amplification polymorphism (MSAP) and high-performance liquid chromatography methods. In F-1, 53.4% fragments were inherited from both A- and C-genomes. Besides, 5.04 and 8.87% fragments in F-1 were inherited from A- and C- genome, respectively. 5.85 and 0.8% fragments were newly appeared and disappeared in resynthesized B. napus, respectively. 13.1% of these gene sites were identified with methylation changes in namely, hypermethylation (7.86%) and hypomethylation (5.24%). The lowest methylation status was detected in F-1 (38.7%) compared with in S-1-S-3. In S-3, 40.32% genes were methylated according to MSAP analysis. Sequencing of methylated fragments indicated that genes involved in multiple biological processes were modified, including transcription factors, protein modification, and transporters. Expression ananlysis of DNA methyltransferase 1 and DNA methyltransferase chromomethylase 3 in different materials was consistent to the DNA methylation status. These results can generally facilitate dissection of how DNA methylation contributes to genetic stability and improvement of B. napus during polyploidization.