Long-Term Memory Formation in Drosophila Requires Training-Dependent Glial Transcription
Long-Term Memory Formation in Drosophila Requires Training-Dependent Glial Transcription
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DOI：
10.1523/jneurosci.3865-14.2015
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发表时间：
2015-04-08
影响因子：
5.3
通讯作者：
Saitoe, Minoru
中科院分区：
文献类型：
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作者：
Matsuno, Motomi;Horiuchi, Junjiro;Saitoe, Minoru
长期记忆（LTM）的形成需要神经元中的从头基因表达，以及随后的突触结构和功能修饰。然而，在这个过程中神经胶质细胞基因表达的重要性尚未得到很好的研究。在这份报告中，我们的特点是细胞粘附分子，克林贡（Klg），这是所需的LTM形成在果蝇。我们发现Klg定位于神经元和胶质细胞之间的接合处，并且在两种细胞类型中的表达是LTM所需的。我们进一步发现，在klg突变体和敲除细胞系中，神经胶质基因repo的表达减少。repo表达是LTM所需的，并且在LTM诱导后表达增加。此外，增加神经胶质中的repo表达足以恢复klg敲低系中的LTM。这些数据表明，神经元活动增强Klg介导的神经元-神经胶质细胞的相互作用，导致神经胶质细胞表达的repo的增加。Repo是一种同源结构域转录因子，表明LTM还需要进一步下游胶质细胞基因表达。
Long-term memory (LTM) formation requires de novo gene expression in neurons, and subsequent structural and functional modification of synapses. However, the importance of gene expression in glia during this process has not been well studied. In this report, we characterize a cell adhesion molecule, Klingon (Klg), which is required for LTM formation in Drosophila. We found that Klg localizes to the juncture between neurons and glia, and expression in both cell types is required for LTM. We further found that expression of a glial gene, repo, is reduced in klg mutants and knockdown lines. repo expression is required for LTM, and expression increases upon LTM induction. In addition, increasing repo expression in glia is sufficient to restore LTM in klg knockdown lines. These data indicate that neuronal activity enhances Klg-mediated neuron-glia interactions, causing an increase in glial expression of repo. Repo is a homeodomain transcription factor, suggesting that further downstream glial gene expression is also required for LTM.