The effect of treatment of cystic fibrosis pulmonary exacerbations on airways and systemic inflammation
The effect of treatment of cystic fibrosis pulmonary exacerbations on airways and systemic inflammation
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DOI：
10.1002/ppul.20646
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发表时间：
2007-08-01
影响因子：
3.1
通讯作者：
Elborn, J. Stuart
中科院分区：
文献类型：
--
作者：
Downey, Damian G.;Brockbank, Simon;Elborn, J. Stuart
背景：囊性纤维化（CF）的慢性感染和气道炎症导致进行性肺损伤中性粒细胞被认为是CF肺内炎症反应的发生和促进的原因。感染和炎症之间的关系是复杂的，但循环炎症标志物可能不能真正反映肺部局部炎症反应。本研究的目的是探讨静脉注射抗生素治疗肺纤维化加重前后痰和血液中炎症生物标志物和细胞的变化。方法：检测包括中性粒细胞弹性酶（NE）和复合物、白细胞介素-8 （IL-8）和可溶性细胞间粘附分子-1 （sICAM-1）、fas配体（fas - l）和TNFr-1。进行痰细胞分化、绝对细胞计数和免疫细胞化学（CD11b和CD95）及分离的血中性粒细胞分析。结果：使用抗生素后，两组患者的绝对或鉴别痰细胞计数或痰溶胶测量无显著差异。最小CD11b染色的中性粒细胞百分比显著增加，平均百分比（SEM）为28(4.1)，而平均百分比（SEM）为41(2.9)，最大染色百分比为30(0.5)，而最大染色百分比为15（2.5）。无CD95染色的中性粒细胞百分比显著增加，平均百分比为43(5.4)，而平均百分比为52（5.1）。结论：这些数据表明静脉注射抗生素可改变全身反应，但局部持续的肺部炎症反应。
Background: Chronic infection in cystic fibrosis (CF) and airway inflammation leads to progressive lung injury Neutrophils are considered to be responsible for the onset and promotion of the inflammatory response within the CF lung. The relationship between infection and inflammation is complex but circulating inflammatory markers may not truly reflect the local inflammatory response in the lung. The aims of this study were to investigate the change of inflammatory biomarkers and cells within sputum and blood before and after intravenous antibiotics for a pulmonary exacerbation of CF Methods: Assays included neutrophil elastase (NE) and complex, interleukin-8 (IL-8) and soluble intercellular adhesion molecule-1 (sICAM-1), fas ligand (FAS-L), and TNFr-1. Analysis of sputum cell differential and absolute cell counts and immunocytochemistry (CD11b and CD95) on sputum and isolated blood neutrophils were carried out. Results: There were no significant differences in absolute or differential sputum cell counts or sputum sol measurements following antibiotics. There was a significant increase in the percentage of blood neutrophils with minimal CD11b staining, 28 (4.1) mean percentage (SEM) versus41 (2.9) and a decrease in the percentage showing maximal staining 30 (0.5) versus 15 (2.5). There was a significant increase in the percentage of blood neutrophils without CD95 staining, 43 (5.4) mean percentage versus 52 (5.1). Conclusion: These data suggest a modifiable systemic response to IV antibiotics but a local sustained inflammatory response in the lung.